Antiproliferative Activities of Acetone Extract From Canarium Odontophyllum (Dabai) Stem Bark Against Human Colorectal Cancer Cells

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Abstract

Colorectal cancer is the most common malignant cancer in developing countries. Canarium odontophyllum, also known as “Dabai” or “Borneo Olive“ is among the natural plants that can potentially be used as an anticancer agent. This study aims to determine the antiproliferative activities and cytotoxicity effects of acetone extract from C. odontophyllum stem bark against human colorectal cancer cell lines HCT 116 and HT 29. Acetone extract of C. odontophyllum stem bark exerted a significant cytotoxic effect on HCT 116 and HT 29 cells determined by MTT assay at the concentration of 12.5 μg/mL to 200 μg/mL for 24, 48, and 72 hours treatment. It was found that acetone extract of C. odontophyllum stem bark inhibited proliferation of HCT 116 with an IC ₅₀ value of 184.93 ± .0 μg/mL, 61.24 ± .1 μg/mL, 79.98 ± .029 for 24, 48 and 72 hours respectively. The findings also showed that acetone extract of C. odontophyllum stem bark revealed a lower inhibitory effect against HT-29 with an IC ₅₀ value of more than 200 μg/mL for 24, 48 and 72 hours. However, acetone extract of C. odontophyllum stem bark at similar concentrations and time points did not show any cytotoxic effect to normal colorectal fibroblast cell CCD18-Co. In conclusion, the acetone extract of C. odontophyllum stem bark exhibited more sensitivity against HCT 116 than HT 29. Its antiproliferative ability towards HCT 116 and HT 29 cells provides insight that this extract may serve as an anticancer agent against colorectal cancer.

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Most cited references 27

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Plant-derived anticancer agents.

John M. Pezzuto (1997)

Natural product drugs play a dominant role in pharmaceutical care. This is especially obvious in the case of antitumor drugs, as exemplified by paclitaxel (Taxol), vincristine (Oncovin), vinorelbine (Navelbine), teniposide (Vumon), and various water-soluble analogs of camptothecin (e.g., Hycamtin). The most efficient method of discovering drugs such as these (i.e. novel chemical prototypes that may function through unique mechanisms of action) is bioactivity-guided fractionation, and it is certain that additional natural product drugs, some of which should be useful for the treatment of humans, remain to be discovered. For the commercial procurement of structurally complex natural product drugs, isolation from plant material may be most practical. With the advent of combinatorial chemistry and high throughput screening, however, even greater progress may now be expected with natural product leads. While systemic drug therapy, to an appreciable extent based on natural products, has been the mainstay of pharmaceutical care, the logic of disease prevention is overwhelming. Bearing in mind the pandemic nature of cancer, a proposal is put forth to create a cancer chemoprevention drug formulation for utilization on a widespread basis by the general population. Cancer chemopreventive agents, many of which are natural products, are capable of preventing or inhibiting the process of carcinogenesis. As with other pharmaceutical agents useful for disease prevention, a pharmacoeconomic analysis of a cancer chemopreventive formulation would need to be considered, and the composition of the formulation should improve over time. Nonetheless, implementation should commence immediately.

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Cytotoxicity, Post-Treatment Recovery, and Selectivity Analysis of Naturally Occurring Podophyllotoxins from Bursera fagaroides var. fagaroides on Breast Cancer Cell Lines

Omar Aristeo Peña-Morán, María Luisa Villarreal, Laura Patricia Alvarez-Berber … (2016)

Despite prevention and treatment options, breast cancer (BC) has become one of the most important issues in the present day. Therefore, the need for more specific and efficient compounds remains paramount. We evaluated four previously isolated aryltetralin lignans: 5′-demethoxy-β-peltatin-A-methylether (1), acetylpodophyllotoxin (2), 5′-demethoxydeoxypodophyllotoxin (3), and 7′,8′-dehydroacetylpodophyllotoxin (4) for cytotoxicity, clonogenicity, and selectivity against three BC cell lines: MCF-7, MDA-MB-231, and BT-549, as well as the non-tumorigenic mammary epithelial cell line MCF-10A. Cytotoxicity was evaluated after 72 h of treatment, and clonogenicity was determined at 72 h post-treatment; experiments were performed using the sulforhodamine B staining assay. Selective-index (SI) was calculated by comparing pure compound IC50 values in MCF-10A cell line against the IC50 of the same compound in cancer cell lines. Structural similarities among lignans and controls (podophyllotoxin and etoposide) were analyzed using the Tanimoto coefficient (Tc). Lignans were cytotoxic against all tested cell lines (0.011–7.22 µM) and clonogenicity testing showed a dose-dependent cytocidality for all lignans (≥0.08 µg/mL); compounds 2 and 3 were more potent (14.1 and 7.6 respectively) than etoposide in BT-549 cell line, while compound 2 displayed selectivity (SI = 28.17) in BT-549 cell line. Tc values of lignans suggested a greater similarity with podophyllotoxin structure.

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Selective cytotoxic activities of two novel synthetic drugs on human breast carcinoma MCF-7 cells.

Ramesh Badisa, Carl B. Goodman, Kate Darling … (2009)

Breast cancer is the second leading cause of cancer deaths in US women. We evaluated two novel compounds, piperidinyl-diethylstilbestrol (DES) and pyrrolidinyl-diethylstilbestrol (DES) for cytotoxicity against brine shrimp larvae, MCF-7 and rat normal liver cells. In vivo cytotoxicity was evaluated against shrimp larvae for 24 h, while in vitro cell toxicity was evaluated by dye binding crystal-violet method after 48 h. The role of these compounds on different phases of the cell cycle was assessed by flow cytometry. In shrimp assay, piperidinyl-DES and pyrrolidinyl-DES were potent with 50% effective dose (ED(50)) values of 7.9+/-0.38 and 15.6+/-1.3 microM, respectively. In MCF-7 and normal liver cells, the 50% lethal concentration (LC(50)) values were 19.7+/-0.95, 17.6+/-0.4 microM and 35.1 and >50 microM, respectively. Cell cycle analyses indicated that MCF-7 cells were arrested at the G(0)/G(1) stage with these compounds. The results indicate that pyrrolidinyl-DES possesses highly selective, potent anticancer activity.

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Author and article information

Journal

Journal ID (nlm-ta): Dose Response

Journal ID (iso-abbrev): Dose Response

Journal ID (hwp): spdos

Journal ID (publisher-id): DOS

Title: Dose-Response

Publisher: SAGE Publications (Sage CA: Los Angeles, CA )

ISSN (Electronic): 1559-3258

Publication date (Electronic): 14 April 2023

Publication date Collection: Apr-Jun 2023

Volume: 21

Issue: 2

Electronic Location Identifier: 15593258221098980

Affiliations

[1 ]Centre for Diagnostic, Therapeutic and Investigative Studies (CODTIS), Faculty of Health Sciences, Ringgold 61775, universityUniversiti Kebangsaan Malaysia; , Kuala Lumpur, Malaysia

[2 ]Faculty of Health Sciences, Ringgold 61775, universityUniversiti Kebangsaan Malaysia; , Kuala Lumpur, Malaysia

[3 ]Center for Healthy Aging and Wellness (H-CARE), Faculty of Health Sciences, Ringgold 61775, universityUniversiti Kebangsaan Malaysia; , Kuala Lumpur, Malaysia

Author notes

[*]Dayang Fredalina Basri, Centre for Diagnostic, Therapeutic and Investigative Studies (CODTIS), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. Email: dayang@ 123456ukm.edu.my

Author information

Siti Fairuz Ishak https://orcid.org/0000-0002-3132-2059

Dayang Fredalina Basri https://orcid.org/0000-0001-6667-9253

Article

Publisher ID: 10.1177_15593258221098980

DOI: 10.1177/15593258221098980

PMC ID: 10108421

SO-VID: f4ba8fa7-0877-442b-9005-2c835b74f2dd

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This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages ( https://us.sagepub.com/en-us/nam/open-access-at-sage).

History

Date received : 15 February 2022

Date accepted : 12 April 2022

Funding

Funded by: Research University Grant;

Award ID: GUP-2016-036

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typesetter ts10

cover-date April-June 2023

Keywords: canarium odontophyllum,colorectal cancer,antiproliferation,stem bark,cytotoxicity,bioactivity,hct 116 cell line,ht 29 cell line

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Keywords: canarium odontophyllum, colorectal cancer, antiproliferation, stem bark, cytotoxicity, bioactivity, hct 116 cell line, ht 29 cell line

Antiproliferative Activities of Acetone Extract From Canarium Odontophyllum (Dabai) Stem Bark Against Human Colorectal Cancer Cells

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Abstract

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Stress signalling in stem cells

Most cited references 27

Plant-derived anticancer agents.

Cytotoxicity, Post-Treatment Recovery, and Selectivity Analysis of Naturally Occurring Podophyllotoxins from Bursera fagaroides var. fagaroides on Breast Cancer Cell Lines

Selective cytotoxic activities of two novel synthetic drugs on human breast carcinoma MCF-7 cells.

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