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      EVALUACIÓN DE DIETAS MERÍDICAS PARA LA CRÍA EN LABORATORIO DE Spodoptera frugiperda (J.E. SMITH) (LEPIDOPTERA: NOCTUIDAE) Translated title: EVALUATION OF MERIDIC DIETS FOR REARING Spodoptera frugiperda (J.E. SMITH) (LEPIDOPTERA: NOCTUIDAE) UNDER LABORATORY CONDITIONS

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          Abstract

          Spodoptera frugiperda es uno de los insectos omnívoros de mayor importancia en el neotrópico americano. Ataca una amplia variedad de cultivos, entre los cuales, se destacan el algodonero y el maíz. De ambas especies existen cultivares transgénicos propuestos como herramientas en su control. Para evaluar su eficacia y monitorear la posible resistencia de insectos a través del tiempo a las toxinas incorporadas, se deben retomar las investigaciones de técnicas de cría con dietas merídicas, a las que se pueden incorporar toxinas del Bacillus thuringiensis y que, además, proporcionan las condiciones nutricionales requeridas y reducen los costos. Esta investigación buscó definir una dieta artificial adecuada para la cría del cogollero del maíz, útil para monitorear su posible resistencia a cultivares transgénicos. La población base, se colectó sobre maíz en el Municipio de Anolaima, Cundinamarca y su desarrollo, se llevó durante tres generaciones consecutivas, sobre las dietas ICRISAT Diet 3, Greene modificada y Shorey & Hale modificada y alimento natural, hojas de maíz. Con todas las dietas, se logró el desarrollo, pero se presentaron diferencias en la duración del ciclo, en la supervivencia y en el peso de larvas y pupas. La dieta que presentó los mejores resultados fue la dieta ICRISAT, al proporcionar a las larvas una menor duración de desarrollo, un menor número de instares, los mayores pesos de larvas y de pupas, y una supervivencia cercana al 100%. Por esta razón, se sugiere el empleo de la dieta ICRISAT en estudios toxicológicos, donde se requiera la incorporación de las toxinas Cry.

          Translated abstract

          Spodoptera frugiperda is one of the omnivorous insects of mayor importance in the American neo-tropic, due to its attacks of many crops, specially cotton and corn. For both crops, transgenic cultivars are proposed as a tool of pest management. In order to determine the efficacy of Bt crops and to monitor possible insect resistance through time to the incorporated toxins, research regarding insect rearing on meridic diets, in which Bacillus thuringiensis toxins can be incorporated and furthermore provide the required nutritional conditions and reduce costs, has to be evaluated. This study sought to define an adequate artificial diet for rearing the fall armyworm, which meets the above mentioned characteristics. An initial S. frugiperda population was collected at Anolaima (Department of Cundinamarca) and its development was studied, through three consecutive generations, on ICRISAT Diet 3, modified Greene, modified Shorey & Hale diet and corn leaves as natural food. With all diets the insect´s development was attained, but differences in life cycle duration, survival and larval and pupal weight were observed. Best results were obtained with the ICRISAT diet: larvae presented a shorter developmental time, a minor number of instars, and higher weights, for larvae and pupae. Its survival was nearly 100%. For this reason, the use of ICRISAT diet for toxicological studies, where the incorporation of a Cry toxin is required, is suggested.

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          Most cited references36

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          Integrated pest management: historical perspectives and contemporary developments.

          M Kogan (1998)
          Twenty five years after its first enunciation, IPM is recognized as one of the most robust constructs to arise in the agricultural sciences during the second half of the twentieth century. The history of IPM, however, can be traced back to the late 1800s when ecology was identified as the foundation for scientific plant protection. That history, since the advent of modern organosynthetic pesticides, acquired elements of drama, intrigue, jealousy, and controversy that mark the path of many great scientific or technological achievements. Evolution of IPM followed multiple paths in several countries and reached beyond the confines of entomological sciences. Time and space constraints, however, bias this review toward entomology, among the plant protection sciences, and give it an obvious US slant, despite the global impact of IPM.
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            Integrated pest management: Historical perspectives and contemporary developments

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              Rearing of Two Lepidopterous Pests of Rice on a Common Artificial Diet

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                Author and article information

                Journal
                rudca
                Revista U.D.C.A Actualidad & Divulgación Científica
                rev.udcaactual.divulg.cient.
                Universidad de Ciencias Aplicadas y Ambientales (Bogotá, Distrito Capital, Colombia )
                0123-4226
                June 2009
                : 12
                : 1
                : 91-100
                Affiliations
                [01] orgnameUniversidad de Ciencias Aplicadas y Ambientales orgdiv1Facultad de Ingeniería Agronómica
                [02] orgnameUniversidad de Ciencias Aplicadas y Ambientales orgdiv1Facultad de Ingeniería Agronómica
                Article
                S0123-42262009000100009 S0123-4226(09)01200109
                fa285712-8961-4e10-9c5a-c58fde7078be

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 27, Pages: 10
                Product

                SciELO Colombia

                Categories
                Ciencias Agropecuarias y Biológicas.

                supervivencia,toxinas Cry,bioensayos,técnicas de cría,Cogollero del maíz,survival,Cry toxins,bioassays,rearing techniques,Fall armyworm

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