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      Early line and hook fishing at the Epipaleolithic site of Jordan River Dureijat (Northern Israel)

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          Abstract

          Nineteen broken and complete bone fish hooks and six grooved stones recovered from the Epipaleolithic site of Jordan River Dureijat in the Hula Valley of Israel represent the largest collection of fishing technology from the Epipaleolithic and Paleolithic periods. Although Jordan River Dureijat was occupied throughout the Epipaleolithic (~20–10 kya the fish hooks appear only at the later stage of this period (15,000–12,000 cal BP). This paper presents a multidimensional study of the hooks, grooved stones, site context, and the fish assemblage from macro and micro perspectives following technological, use wear, residue and zooarchaeological approaches. The study of the fish hooks reveals significant variability in hook size, shape and feature type and provides the first evidence that several landmark innovations in fishing technology were already in use at this early date. These include inner and outer barbs, a variety of line attachment techniques including knobs, grooves and adhesives and some of the earliest evidence for artificial lures. Wear on the grooved stones is consistent with their use as sinkers while plant fibers recovered from the grooves of one hook shank and one stone suggest the use of fishing line. This together with associations between the grooved stones and hooks in the same archaeological layers, suggests the emergence of a sophisticated line and hook technology. The complexity of this technology is highlighted by the multiple steps required to manufacture each component and combine them into an integrated system. The appearance of such technology in the Levantine Epipaleolithic record reflects a deep knowledge of fish behavior and ecology. This coincides with significant larger-scale patterns in subsistence evolution, namely broad spectrum foraging, which is an important first signal of the beginning of the transition to agriculture in this region.

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          In situ immune response and mechanisms of cell damage in central nervous system of fatal cases microcephaly by Zika virus

          Zika virus (ZIKV) has recently caused a pandemic disease, and many cases of ZIKV infection in pregnant women resulted in abortion, stillbirth, deaths and congenital defects including microcephaly, which now has been proposed as ZIKV congenital syndrome. This study aimed to investigate the in situ immune response profile and mechanisms of neuronal cell damage in fatal Zika microcephaly cases. Brain tissue samples were collected from 15 cases, including 10 microcephalic ZIKV-positive neonates with fatal outcome and five neonatal control flavivirus-negative neonates that died due to other causes, but with preserved central nervous system (CNS) architecture. In microcephaly cases, the histopathological features of the tissue samples were characterized in three CNS areas (meninges, perivascular space, and parenchyma). The changes found were mainly calcification, necrosis, neuronophagy, gliosis, microglial nodules, and inflammatory infiltration of mononuclear cells. The in situ immune response against ZIKV in the CNS of newborns is complex. Despite the predominant expression of Th2 cytokines, other cytokines such as Th1, Th17, Treg, Th9, and Th22 are involved to a lesser extent, but are still likely to participate in the immunopathogenic mechanisms of neural disease in fatal cases of microcephaly caused by ZIKV.
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            Stable Isotope Evidence for Similarities in the Types of Marine Foods Used by Late Mesolithic Humans at Sites Along the Atlantic Coast of Europe

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              The Tortoise and the Hare

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                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: MethodologyRole: Project administrationRole: VisualizationRole: Writing – original draft
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: VisualizationRole: Writing – original draft
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: MethodologyRole: SoftwareRole: Writing – original draft
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: Funding acquisitionRole: MethodologyRole: Project administrationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                6 October 2021
                2021
                : 16
                : 10
                : e0257710
                Affiliations
                [1 ] TraCEr, Laboratory for Traceology and Controlled Experiments at MONREPOS Archaeological Research Centre and Museum for Human Behavioural Evolution, RGZM, Mainz, Germany
                [2 ] DANTE–Diet and Ancient Technology Laboratory, Department of Oral and Maxillo-Facial Sciences, Sapienza University of Rome, Roma, Italy
                [3 ] Department of Anthropology, University of Connecticut, Storrs Mansfield, Connecticut, United States of America
                [4 ] Institute of Archaeology, The Hebrew University of Jerusalem, Jerusalem, Israel
                [5 ] Department of Galilee Studies (M.A.), Tel Hai College, Qiryat Shemona, Israel
                Universita degli Studi di Ferrara, ITALY
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                https://orcid.org/0000-0002-1226-2556
                Article
                PONE-D-21-11773
                10.1371/journal.pone.0257710
                8494375
                34613991
                fb438d78-ae64-411f-9344-5cfdad6b1681
                © 2021 Pedergnana et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 9 April 2021
                : 9 September 2021
                Page count
                Figures: 14, Tables: 3, Pages: 38
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/501100003977, Israel Science Foundation;
                Award ID: Grant #918/17
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/501100000781, European Research Council;
                Award ID: 639286
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/100000001, National Science Foundation;
                Award ID: BCS-1842087
                Award Recipient :
                Research is supported by the Israel Science Foundation - www.isf.org.il (Grant #918/17) granted to G. Sharon. This research was also supported by grants from the European Research Council - https://erc.europa.eu (Starting Grant Project HIDDEN FOODS, grant no. 639286) awarded to E. Cristiani and the National Science Foundation - https://www.nsf.gov (BCS-1842087) awarded to N. Munro. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
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                Social Sciences
                Archaeology
                Biology and Life Sciences
                Organisms
                Eukaryota
                Animals
                Vertebrates
                Fish
                Freshwater Fish
                Biology and Life Sciences
                Zoology
                Animals
                Vertebrates
                Fish
                Freshwater Fish
                Earth Sciences
                Mineralogy
                Minerals
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                Organisms
                Eukaryota
                Animals
                Vertebrates
                Fish
                Marine Fish
                Biology and Life Sciences
                Zoology
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                Vertebrates
                Fish
                Marine Fish
                Biology and Life Sciences
                Marine Biology
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                Earth Sciences
                Marine and Aquatic Sciences
                Marine Biology
                Marine Fish
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                Archaeological Dating
                Custom metadata
                All archaeological materials (fish hooks, grooved stones, fish bones) are curated in the Department of Galilee Studies at Tel Hai College, Israel. All relevant data on the fish hooks and grooved stones are published within the paper. An inventory of the bone hooks and grooved stones including catalog numbers are indicated in Table 1 and Table 3. The fauna are too numerous to list individually by number, but the data presented in Figure 14 are available in an online repository [ https://doi.org/10.7910/DVN/VTKRQW] as are the 3D models of the hooks and grooved stones.

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