ABSTRACT The characteristics of wood and charcoal depend on the genetic material and the sampling position on the tree trunk. The objective of this study was to evaluate the effect of clone and longitudinal position on the characteristics of wood and charcoal of Eucalyptus grandis x Eucalyptus urophylla. Specimens belonging to I144 and GG100 clones both at seven years of age were felled and mechanically processed for disc removal at different longitudinal positions of 0, 3, 6, 9, 12 and 15 meters from the ground. The wood and charcoal physical and energetic characteristics were evaluated according to the different longitudinal positions. The genetic material and longitudinal position influenced the wood and charcoal characteristics. Sampling positions on the tree trunks can be considered for the energetic direction of “in natura” or carbonized biomass.