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      Digital lensless holographic microscopy and applications Translated title: Microscopia holográfica digital sin lentes y aplicaciones

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          Abstract

          Abstract The principles and some applications of the digital lensless holographic microscopy (DLHM) to study the microworld are shown in this paper. The recording and reconstruction processes of the DLHM are analyzed to study its lateral resolution power. The effect of the spatial coherence in the study of a section of the head of a Drosophila melanogaster fly are presented. DLHM is applied to study dwynamic and static colloidal systems as a proof of the capability of achieving micrometer spatial lateral resolution without the use of lenses.

          Translated abstract

          Resumen En este artículo se discuten los principios y algunas aplicaciones de la microscopia holográfica digital sin lentes (MHDSL) como una herramienta para el estudio del micromundo. Se analizan los procesos de registro y reconstrucción de MHDSL para estudiar su poder de resolución lateral. Se presenta el efecto de la coherencia espacial en el estudio de una sección de la cabeza de una mosca Drosophila Melanogaster por medio de la MHDSL. Se aplica la MHDSL al estudio de sistemas coloidales dinámicos y estáticos como una prueba de la capacidad de resolución espacial micrométrica sin la necesidad del uso de lentes.

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          Most cited references22

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          A new microscopic principle.

          D. Gabor (1948)
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            Microscopy by Reconstructed Wave-Fronts

            D. Gabor (1949)
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              Digital holography for quantitative phase-contrast imaging.

              We present a new application of digital holography for phase-contrast imaging and optical metrology. This holographic imaging technique uses a CCD camera for recording of a digital Fresnel off-axis hologram and a numerical method for hologram reconstruction. The method simultaneously provides an amplitude-contrast image and a quantitative phase-contrast image. An application to surface profilometry is presented and shows excellent agreement with contact-stylus probe measurements.
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                Author and article information

                Contributors
                Role: ND
                Journal
                inco
                Ingeniería y competitividad
                Ing. compet.
                Facultad de Ingeniería, Universidad del Valle (Cali, Valle del Cauca, Colombia )
                0123-3033
                June 2017
                : 19
                : 1
                : 129-137
                Affiliations
                [1] Medellin orgnameUniversidad Nacional de Colombia orgdiv1Escuela de Física Colombia jigarcía@ 123456unal.edu.co
                Article
                S0123-30332017000100129
                fff5fa8a-94cc-40fb-94ba-e60169fca7f5

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 26 November 2015
                : 16 August 2016
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 22, Pages: 9
                Product

                SciELO Colombia


                Colloidal systems,digital lens less holographic microscopy,spatial coherence,Coherencia espacial parcial,microscopía holográfica digital sin lentes,sistemas coloidales

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