AstraZeneca's covid-19 vaccine demonstrates some concerns regarding its excipients, such as polysorbate 80, which serves as a stabilizer, and EDTA, which serves as a binding agent. 7
ADA is a critical purine metabolic enzyme required for adequate immunological competence. The current study examines the effect of Zn2+ on the maintenance of ADA. After eliminating Zn2+, the protein's crystallographic structure has retained a high degree of order and similarity to the original one, with some alterations limited to the areas surrounding the pocket. However, the protein's stability has been dramatically reduced.
EDTA is a highly effective zinc-chelating agent employed in the investigations of protein interactions. The potential damage of the structure and interactions of zinc-binding proteins by EDTA treatment must be considered. Excess Zn2+ has not returned C/H1 to its normal structure, highlighting the irreversible denaturation induced by the EDTA.
Around 30% of proteins in cells form complexes with metals. Metal ions are essential not just for regulating biological functions but also for stabilizing proteins. (ADA, EC 18.104.22.168) has a molecular mass of 40 kDa and contains a firmly attached zinc ion essential for its activity. The lack of its enzymatic activity leads to malfunction B and T cells, and SCID development