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      The Emergence of the Tetrathionate Reductase Operon in the Escherichia coli/Shigella Pan-genome.

      Preprint
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      research-article
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      ScienceOpen Preprints
      ScienceOpen
      E. coli, Shigella, tetrathionate reductase
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            Abstract

            Escherichia coli pathogenic variants (pathovars) are generally characterized by defined virulence traits and are susceptible to the evolution of hybridized identities due to the considerable plasticity of the E. coli genome. We have isolated a strain from a purified diet intended for research animals that further demonstrates the ability of E. coli to acquire novel genetic elements leading potentially to emergent new pathovars. Utilizing Next Generation Sequencing (NGS) to obtain a Whole Genome (WGS) profile, we report an atypical strain of E. coli, EcoFA807-17, possessing a tetrathionate reductase ( ttr) operon, which enables the utilization of tetrathionate as an electron acceptor, thus facilitating respiration in anaerobic environments such as the mammalian gut. The ttr operon is a potent virulence factor for several enteric pathogens, most prominently Salmonella enterica. However, the presence of chromosomally integrated tetrathionate reductase genes do not appear to have been previously reported in wild-type E. coli or Shigella. Accordingly, it is possible that the appearance of this virulence factor may signal the evolution of new mechanisms of pathogenicity in E. coli and Shigella, and may potentially alter the effectiveness of existing assays using tetrathionate reductase as a unique marker for the detection of Salmonella enterica.

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            Author and article information

            Journal
            ScienceOpen Preprints
            ScienceOpen
            17 June 2022
            Affiliations
            [1 ] National Institute of Environmental Health Sciences/Comparative Medicine Branch/Quality Assurance Laboratory
            Author notes
            Article
            10.14293/S2199-1006.1.SOR-.PP9VYWN.v1
            4d0436b4-0c47-4f8c-8553-c0135b49f8ac

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

            Funding
            National Institute of Environmental Health Sciences N/A - In-house funding

            The datasets generated during and/or analysed during the current study are available in the repository: https://www.ncbi.nlm.nih.gov/bioproject/721428
            Microbiology & Virology,Life sciences
            E. coli,Shigella,tetrathionate reductase

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