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      Next-generation sequencing with emphasis on Illumina and Ion torrent platforms.

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            Abstract

            Abstract

            Background: Next-generation sequencing is a type of deep sequencing. In comparison to the previously used Sanger's method, Next generation sequencing allowing the sequencing of an entire genome in a single day. Next-generation sequencing (NGS) has revolutionized genomics and molecular biology. NGS has a wide range of medical applications, including tumors and inherited disease diagnosis. It is also used to find genetic variants across the genome. There are several NGS platforms available. Illumina and Iontorrent are the most prevalent sequencing platforms. These NGS platforms reduced the cost and time required to sequence a full genome.

            The main body of the abstract: The review paper covered a brief history of next-generation sequencing technology (NGS), followed by the benefits of employing this revolutionary technology and The general approach of NGS, beginning with fragmentation and ending with data analysis, was explained, with a focus on the Illumina and Ion torrent platforms. Finally, the data analysis step was thoroughly covered, beginning with data quality control and ending with data visualization. Conclusion: According to the review article, Next generation sequencing (NGS) is a promising technology that has revolutionized genome sequencing. The NGS platform has resulted in softwares that can perform the vast majority of NGS steps such as sequencing, variant annotation, and quality checks. The Iontorrent and Illumina platforms have grown in popularity and are frequently used. NGS has gained traction in clinical applications.

            Conclusion: According to the review article, Next generation sequencing (NGS) is a promising technology that has revolutionized genome sequencing. The NGS platform has resulted in softwares that can perform the vast majority of NGS steps such as sequencing, variant annotation, and quality checks. The Iontorrent and Illumina platforms have grown in popularity and are frequently used. NGS has gained traction in clinical applications.

            Content

            Author and article information

            Journal
            ScienceOpen Preprints
            ScienceOpen
            24 August 2022
            Affiliations
            [1 ] Department of Microbiology, Faculty of science, Alexandria University, Egypt.
            [2 ] Faculty of medicine, El-Qalam College for science and technology, Sudan.
            Author notes
            Article
            10.14293/S2199-1006.1.SOR-.PPA9N9O.v1
            5c2248f7-f021-4769-8fdd-352b06542234

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .


            Data sharing not applicable to this article as no datasets were generated or analysed during the current study.
            Molecular medicine,Molecular biology

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