Nuts and seeds are good sources of oil. Most of these oil are edible and are of industrial use. In term of edibility, some seed oils are hyperlipidemic, while some are hypolipidemic. The Hura crepitans (sandbox tree) is one of the plants that bear seeds however, the seeds has not been fully utilized. This study investigated the effects of Hura crepitans seed oil on lipidomics of some tissues (blood, liver, heart, kidney and brain) in albino rats. The Hura crepitans seed oil (HCSO) was extracted with Soxhlet extractor using petroleum ether as solvent. Twenty-four male albino rats allocated to four groups of 6 rats each were used. Groups 1-3 were fed diet containing 5 %, 10 % and 15 % HCSO respectively while group 4 was fed diet containing 15% groundnut oil (GO) (control) for 8 weeks. Whole blood was collected and the tissues (liver, heart, brain and kidney) were harvested. Cholesterol, triacylglycerol and phospholipid levels were determined in plasma, RBC and tissues of the rats spectrophotometrically. Hepatic HMG-CoA reductase activity was indirectly determined using spectrophotometric method. One-way analysis of variance (ANOVA) and Tukeys test were used to analyse the results. The lipids analyses showed that HDL and VLDL cholesterol levels decreased significant (p < 0.05) in the 5 % and 15 % HCSO groups and in plasma, liver and kidney of 10% HCSO group. Also a significantly ( p< 0.05) increase was observed in RBC cholesterol level in 5 % and 15 % HCSO groups while cardiac cholesterol level increased significantly in 10 % HCSO group. Triacylglycerol level increased significantly in plasma, VLDL and liver (5 % and 10% HCSO group), kidney and brain (5 % HCSO group), RBC and heart (15 % HCSO group) while a decrease ensued in HDL across the groups. The phospholipids level decreased significantly in all the organs except kidney of 15 % HCSO group whereas an increase was observed in plasma, HDL, VLDL and RBC of all the groups. Hura crepitans seed oil is considerably hypolipidemic and could be edible and be beneficial to health especially at 10 % composition.