Aflatoxins B1, B2, G1 and G2 were detected in all cowpea samples analyzed in this study. Samples collected from Oja-Oba market were found to contain the highest amount Aflatoxins.Cowpea is an essential crop with multipurpose usage among the locals of Nigeria, which made the country famous (1st) in both production and consumption. However, most seeds (cowpea seeds inclusive) are highly susceptible to microbial contamination under poor storage conditions (Etaware and Etaware, 2019). Aflatoxins were reportedly detected in the kidney of 58% of deceased children caused by kwashiorkor at Obafemi Awolowo University Teaching Hospital, Ile-Ife (Ogungbemile et al., 2020).The laboratory analysis conducted showed that Aflatoxins B1, B2, G1 and G2 were detected in all the cowpea samples collected from Sasa, Bodija and Oja-Oba markets in Ibadan, Oyo State, Nigeria.