Phytochemical Analysis and Antimicrobial Activity of Selected Medicinal Plants.

Background:

Asteracantha longifolia , P. tuberosa and M. pruriens are traditionally being use for medicinal purposes in India. Present study was designed to check in-vitro efficacy of these plants against selected bacterial strains.

Methodology:

Methanol extracts of these plants were used for Phytochemical Analysis to check the presence of different phytochemical compounds such as Saponins, Tannins, Flavonoids, Amino acid, Carbohydrates and various other compounds. Antibacterial activity of these two plant extract were tested against two pathogenic bacterial strains i.e. Escherichia coli, Bacillus Subtilis and efficiency of these plant extract were compared against three antibiotics at their standard concentrations viz. Gentamycin (50mg/ml), Tetracycline(30mg/ml), Chloramphenicol(34mg/ml).

Results:

Phytochemical analysis of A.longifolia , P. tuberosa and M. pruriens confirmed the presence of various phytochemical compounds such as Saponins, Tannins, Flavonoids, Amino acid and Carbohydrates.

It was observed that the plant extract showed no inhibitory effect against the two selected bacterial strains i.e. Escherichia coli, Bacillus Subtilis. While three antibiotics showed considerable inhibitory effect against the two bacterial strains which was confirmed by the zone of inhibition.