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      Intensification of biokinetics of enzymes using ultrasound assisted methods: A critical review

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          Abstract

          The use of low intensity ultrasound has gotten surprising consideration over the last decade as a method for enhancing the catalytic activity of enzyme. Ultrasounds have the potential to significantly influence the activity of the enzymatic processes, provided that the energy input is not too high to inactivate the enzyme. By providing the variation in parameters, various physical and chemical effects can be attained that can enhance the enzymatic reaction. Ultrasonic reactors are known for their application in bioprocesses. However, the potential of their applications is still limited broadly due to the lack of proper information about their operational and performance parameters. In this review, the detailed information about ultrasonic reactors is provided by defining the different types of reactors, number and position of ultrasonic transducers. Also, it includes mechanism of intensification and influence of ultrasonic parameters (intensity, duty cycle and frequency) and enzymatic factors (enzyme concentration, temperature and pH) on the catalytic activity of enzyme during ultrasound treatment.

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          Author and article information

          Journal
          ScienceOpen Preprints
          ScienceOpen
          9 January 2021
          Affiliations
          [1 ] DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, 400019, Maharashtra, India
          [2 ] Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, Mumbai, 400019, Maharashtra, India
          Article
          10.14293/S2199-1006.1.SOR-.PPGGTWU.v1

          This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .

          Data sharing not applicable to this article as no datasets were generated or analysed during the current study.

          Biochemistry, Catalysis, Biomedical engineering, Biophysics

          Ultrasound, Sonochemical reaction, Enzymatic reaction, Enzyme kinetic, Ultrasonic reactors

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