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      Isolation and Identification of Fungi Associated with the Spoilage of Sweet Orange and Banana in Sokoto Metropolis

      Preprint
      In review
      research-article
        1 ,
      ScienceOpen Preprints
      ScienceOpen
      Mycology, Fungi, Pathogenecity, Isolation, Identification, Sweet orange, Banana, Spoilage
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            Abstract

            Mycological studies on Fungi in apparently diseased Sweet Orange ( Citrus sinensis) and Banana ( Musa sapientum) sampled from various points in Kara market in Sokoto Metropolis was carried out between August and September. The samples were surfaced sterilised with ethanol and the homogenates were cultured on Potato Dextrose Agar (PDA) and incubated aerobically at room temperature for 7 days at 30C. The pure cultures obtained were identified morphologically and microscopically. The investigation revealed that the samples were infected with several fungi species. The most predominant Fungi isolated from Sweet Orange were Cladosporium spp (40%), Fusarium spp (30%), Alternaria spp ( 20%), and Chrysonilia spp (10%) while the most predominant Fungi isolated from Banana were Fusarium spp (50%), Mucor spp ( 30%) and Rhizopus spp (20%). The pathogenecity test results show that Cladosporium spp and Fusarium spp were the most active in sweet orange with rot length of 74 mm and 70 mm respectively and the least active fungi were Alternaria spp and Chrysonilia spp with rot lengths of 52 mm and 48 mm respectively. Furthermore, Fusarium spp and Mucor spp were the most active in banana with rot lengths of 84 mm and 75 mm respectively and the least active fungus was Rhizopus spp with rot lengths of 54 mm.

            Content

            Author and article information

            Journal
            ScienceOpen Preprints
            ScienceOpen
            3 November 2020
            Affiliations
            [1 ] Department of Crop Science, Usman Danfodiyo University, Sokoto
            Article
            10.14293/S2199-1006.1.SOR-.PPN39LD.v1
            c74c41e0-f688-4c4c-b00c-d78494823a75

            This work has been published open access under Creative Commons Attribution License CC BY 4.0 , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com .


            All data generated or analysed during this study are included in this published article (and its supplementary information files).
            Agriculture
            Mycology,Spoilage,Banana,Sweet orange,Identification,Isolation,Pathogenecity,Fungi

            Comments

            The article though a preliminary research is indeed interesting. The Author Micheal Ameh, identified and gave the distribution of fungi spp affecting sweet orange and banana in the study area. He went further to identify the most active of these spp based on pathogenicity test. This finding will be a good platform for advanced mycological studies such as what on-farm or off-farm preventive or control measure will be most effective on the identified fungi spp in the study area.  

            Samuel Ipinyomi 

            2020-11-16 13:33 UTC
            +1

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