48
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Molecular interplay of the noncoding RNA ANRIL and methylated histone H3 lysine 27 by polycomb CBX7 in transcriptional silencing of INK4a.

      Molecular Cell

      Animals, Cell Aging, physiology, Cell Line, Tumor, Cyclin-Dependent Kinase Inhibitor p15, genetics, Cyclin-Dependent Kinase Inhibitor p16, metabolism, Gene Silencing, Histones, Humans, Lysine, Male, Methylation, Models, Molecular, Molecular Sequence Data, Multigene Family, Nuclear Magnetic Resonance, Biomolecular, Polycomb Repressive Complex 1, Polycomb-Group Proteins, Prostatic Neoplasms, pathology, Protein Structure, Tertiary, RNA, Untranslated, Repressor Proteins, chemistry, Transcription, Genetic

      Read this article at

      ScienceOpenPublisherPMC
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Expression of the INK4b/ARF/INK4a tumor suppressor locus in normal and cancerous cell growth is controlled by methylation of histone H3 at lysine 27 (H3K27me) as directed by the Polycomb group proteins. The antisense noncoding RNA ANRIL of the INK4b/ARF/INK4a locus is also important for expression of the protein-coding genes in cis, but its mechanism has remained elusive. Here we report that chromobox 7 (CBX7) within the polycomb repressive complex 1 binds to ANRIL, and both CBX7 and ANRIL are found at elevated levels in prostate cancer tissues. In concert with H3K27me recognition, binding to RNA contributes to CBX7 function, and disruption of either interaction impacts the ability of CBX7 to repress the INK4b/ARF/INK4a locus and control senescence. Structure-guided analysis reveals the molecular interplay between noncoding RNA and H3K27me as mediated by the conserved chromodomain. Our study suggests a mechanism by which noncoding RNA participates directly in epigenetic transcriptional repression. Copyright (c) 2010 Elsevier Inc. All rights reserved.

          Related collections

          Author and article information

          Journal
          20541999
          2886305
          10.1016/j.molcel.2010.03.021

          Comments

          Comment on this article