Background. Lifestyle is one of the major factors in the optimal development of systems and functions of human organism and health. The appropriate level of physical activity is the integral component of a healthy lifestyle. Objective. The study was aimed to investigate the level of physical activity of the female students of Medical University. Methods. We applied the International Physical Activity Questionnaire (IPAQ) in the long version as a research method. Results. The study was conducted among 333 female students of Medical University of the first, second and third year of study. Detailed International Physical Activity Questionnaire evaluating physical activity, such as work, housework, sport, and physical activity during leisure time, was completed. Conclusions. The examined female students of medical university are characterized by high level of total physical activity. Place of residence during the studies does not differentiate significantly the level of physical activity of female students.