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      • Record: found
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      Is Open Access

      Particulate matter from both heavy fuel oil and diesel fuel shipping emissions show strong biological effects on human lung cells at realistic and comparable in vitro exposure conditions.

      1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 9 , 9 , 10 , 10 , 11 , 11 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 17 , 17 , 18 , 19 , 20 , 21 , 21 , 22 , 22 , 6 , 22 , 22 , 22 , 23 , 7 , 22 , 6 , 22 , 22 , 22 , 22 , 6 , 23 , 23 , 23 , 22 , 7 , 4 , 1 , 2 , 5
      PloS one
      Public Library of Science (PLoS)

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          Abstract

          Ship engine emissions are important with regard to lung and cardiovascular diseases especially in coastal regions worldwide. Known cellular responses to combustion particles include oxidative stress and inflammatory signalling.

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          Most cited references29

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          Evidence on the impact of sustained exposure to air pollution on life expectancy from China's Huai River policy.

          This paper's findings suggest that an arbitrary Chinese policy that greatly increases total suspended particulates (TSPs) air pollution is causing the 500 million residents of Northern China to lose more than 2.5 billion life years of life expectancy. The quasi-experimental empirical approach is based on China's Huai River policy, which provided free winter heating via the provision of coal for boilers in cities north of the Huai River but denied heat to the south. Using a regression discontinuity design based on distance from the Huai River, we find that ambient concentrations of TSPs are about 184 μg/m(3) [95% confidence interval (CI): 61, 307] or 55% higher in the north. Further, the results indicate that life expectancies are about 5.5 y (95% CI: 0.8, 10.2) lower in the north owing to an increased incidence of cardiorespiratory mortality. More generally, the analysis suggests that long-term exposure to an additional 100 μg/m(3) of TSPs is associated with a reduction in life expectancy at birth of about 3.0 y (95% CI: 0.4, 5.6).
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            Mortality from ship emissions: a global assessment.

            Epidemiological studies consistently link ambient concentrations of particulate matter (PM) to negative health impacts, including asthma, heart attacks, hospital admissions, and premature mortality. We model ambient PM concentrations from oceangoing ships using two geospatial emissions inventories and two global aerosol models. We estimate global and regional mortalities by applying ambient PM increases due to ships to cardiopulmonary and lung cancer concentration-risk functions and population models. Our results indicate that shipping-related PM emissions are responsible for approximately 60,000 cardiopulmonary and lung cancer deaths annually, with most deaths occurring near coastlines in Europe, East Asia, and South Asia. Under current regulation and with the expected growth in shipping activity, we estimate that annual mortalities could increase by 40% by 2012.
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              • Article: not found

              Transformation of human bronchial epithelial cells by infection with SV40 or adenovirus-12 SV40 hybrid virus, or transfection via strontium phosphate coprecipitation with a plasmid containing SV40 early region genes.

              Normal human bronchial epithelial cells were infected with SV40 virus or an adenovirus 12-SV40 hybrid virus, or transfected via strontium phosphate coprecipitation with plasmids containing the SV40 early region genes. Colonies of morphologically altered cells were isolated and cultured; these cells had extended culture lifespans compared to normal human bronchial epithelial cells. All cultures eventually underwent senescence, with the exception of one which appears to have unlimited proliferative potential. Colonies arising after viral infection were screened for virus production by cocultivation with Vero cells; only viral nonproducer cultures were analyzed further. The cells retained electron microscopic features of epithelial cells, and keratin and SV40 T-antigen were detected by indirect immunofluorescence. All of the cultures were aneuploid with karyotypic abnormalities characteristic of SV40-transformed cells. No tumors formed after s.c. injection of the cells in nude mice. These cells should be useful for studies of multistage bronchial epithelial carcinogenesis.
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                Author and article information

                Journal
                PLoS ONE
                PloS one
                Public Library of Science (PLoS)
                1932-6203
                1932-6203
                2015
                : 10
                : 6
                Affiliations
                [1 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Member of the German Center for Lung Research (DZL), Munich, Germany; CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland.
                [2 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Mass Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine Berlin-Buch, Germany.
                [3 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland.
                [4 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Esch-Belval, Luxembourg.
                [5 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Joint Mass Spectrometry Centre, Chair of Analytical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany; Joint Mass Spectrometry Centre, CMA-Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany.
                [6 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Joint Mass Spectrometry Centre, CMA-Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany.
                [7 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Joint Mass Spectrometry Centre, Chair of Analytical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany.
                [8 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany; Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany.
                [9 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany.
                [10 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany.
                [11 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Chair of Piston Machines and Internal Combustion Engines, University Rostock, Rostock, Germany.
                [12 ] University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland.
                [13 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland; VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Espoo, Finland.
                [14 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland; National Institute for Health and Welfare, Department of Environmental Health, P.O. Box 95, FI-70701, Kuopio, Finland.
                [15 ] Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Member of the German Center for Lung Research (DZL), Munich, Germany.
                [16 ] CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland; Institute of environmental medicine, UNIKA-T, Technische Universität, Munich, Germany.
                [17 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Lung and Particle Research Group, School of Biosciences, Cardiff University, Cardiff, Wales, United Kingdom.
                [18 ] Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München-German Research Center for Environmental Health GmbH, Neuherberg, Germany.
                [19 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Vitrocell GmbH, Waldkirch, Germany.
                [20 ] Laboratory of Atmospheric Chemistry, Paul Scherrer Institute (PSI), Villigen, Switzerland.
                [21 ] HICE-Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health-Aerosols and Health, www.hice-vi.eu, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany, Kuopio, Finland, Cardiff, UK, Esch-Belval, Luxembourg; Institute of Physics, University Rostock, Rostock, Germany.
                [22 ] Joint Mass Spectrometry Centre, CMA-Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany.
                [23 ] Joint Mass Spectrometry Centre, Chair of Analytical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany.
                Article
                PONE-D-14-45336
                10.1371/journal.pone.0126536
                4454644
                26039251
                bd427413-dcb0-4c48-8b05-dc409fa1329d
                History

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