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      First person – Alicia Estacio-Gómez

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      Biology Open
      The Company of Biologists Ltd

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          ABSTRACT

          First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Alicia Estacio-Gómez is first author on ‘ Dynamic neurotransmitter specific transcription factor expression profiles during Drosophila development ’, published in BiO. Alicia is a research associate (postdoc) in the lab of Tony D. Southall at the Department of Life Sciences, Imperial College London, London, UK, investigating neural specification during development and gene regulation.

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          Dynamic neurotransmitter specific transcription factor expression profiles during Drosophila development

          ABSTRACT The remarkable diversity of neurons in the nervous system is generated during development, when properties such as cell morphology, receptor profiles and neurotransmitter identities are specified. In order to gain a greater understanding of neurotransmitter specification we profiled the transcription state of cholinergic, GABAergic and glutamatergic neurons in vivo at three developmental time points. We identified 86 differentially expressed transcription factors that are uniquely enriched, or uniquely depleted, in a specific neurotransmitter type. Some transcription factors show a similar profile across development, others only show enrichment or depletion at specific developmental stages. Profiling of Acj6 (cholinergic enriched) and Ets65A (cholinergic depleted) binding sites in vivo reveals that they both directly bind the ChAT locus, in addition to a wide spectrum of other key neuronal differentiation genes. We also show that cholinergic enriched transcription factors are expressed in mostly non-overlapping populations in the adult brain, implying the absence of combinatorial regulation of neurotransmitter fate in this context. Furthermore, our data underlines that, similar to Caenorhabditis elegans, there are no simple transcription factor codes for neurotransmitter type specification. This article has an associated First Person interview with the first author of the paper.
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            Author and article information

            Journal
            Biol Open
            Biol Open
            BIO
            biolopen
            Biology Open
            The Company of Biologists Ltd
            2046-6390
            15 May 2020
            3 June 2020
            3 June 2020
            : 9
            : 5
            : bio053264
            Article
            BIO053264
            10.1242/bio.053264
            7286288
            058ad090-8e1a-40cc-b8dd-3b77a24a326a
            © 2020. Published by The Company of Biologists Ltd

            This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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            Life sciences
            Life sciences

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