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      Machine Learning for Seed Quality Classification: An Advanced Approach Using Merger Data from FT-NIR Spectroscopy and X-ray Imaging

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          Abstract

          Optical sensors combined with machine learning algorithms have led to significant advances in seed science. These advances have facilitated the development of robust approaches, providing decision-making support in the seed industry related to the marketing of seed lots. In this study, a novel approach for seed quality classification is presented. We developed classifier models using Fourier transform near-infrared (FT-NIR) spectroscopy and X-ray imaging techniques to predict seed germination and vigor. A forage grass ( Urochloa brizantha) was used as a model species. FT-NIR spectroscopy data and radiographic images were obtained from individual seeds, and the models were created based on the following algorithms: linear discriminant analysis (LDA), partial least squares discriminant analysis (PLS-DA), random forest (RF), naive Bayes (NB), and support vector machine with radial basis (SVM- r) kernel. In the germination prediction, the models individually reached an accuracy of 82% using FT-NIR data, and 90% using X-ray data. For seed vigor, the models achieved 61% and 68% accuracy using FT-NIR and X-ray data, respectively. Combining the FT-NIR and X-ray data, the performance of the classification model reached an accuracy of 85% to predict germination, and 62% for seed vigor. Overall, the models developed using both NIR spectra and X-ray imaging data in machine learning algorithms are efficient in quickly, non-destructively, and accurately identifying the capacity of seed to germinate. The use of X-ray data and the LDA algorithm showed great potential to be used as a viable alternative to assist in the quality classification of U. brizantha seeds.

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          Most cited references28

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          Seed vigour and crop establishment: extending performance beyond adaptation

          Seeds are central to crop production, human nutrition, and food security. A key component of the performance of crop seeds is the complex trait of seed vigour. Crop yield and resource use efficiency depend on successful plant establishment in the field, and it is the vigour of seeds that defines their ability to germinate and establish seedlings rapidly, uniformly, and robustly across diverse environmental conditions. Improving vigour to enhance the critical and yield-defining stage of crop establishment remains a primary objective of the agricultural industry and the seed/breeding companies that support it. Our knowledge of the regulation of seed germination has developed greatly in recent times, yet understanding of the basis of variation in vigour and therefore seed performance during the establishment of crops remains limited. Here we consider seed vigour at an ecophysiological, molecular, and biomechanical level. We discuss how some seed characteristics that serve as adaptive responses to the natural environment are not suitable for agriculture. Past domestication has provided incremental improvements, but further actively directed change is required to produce seeds with the characteristics required both now and in the future. We discuss ways in which basic plant science could be applied to enhance seed performance in crop production.
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            Near infrared spectroscopy: A mature analytical technique with new perspectives – A review

            Last decade's advances and modern aspects of near infrared spectroscopy are critically examined and reviewed. Innovative instrumentation, highlighted by portable and imaging instruments, chemometrics data multivariate processing, and new and valuable applications are presented and discussed. Because of these advances, this mature analytical technique is continually experiencing renewed interest. The drawbacks and misuses of the technique and its supporting mathematical tools are also addressed. The principal achievements in the field are shown in a critical manner, in order to understand why the technique has found intensive application in the most diverse and modern areas of analytical importance during the last ten years.
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              Recent Applications of Multispectral Imaging in Seed Phenotyping and Quality Monitoring—An Overview

              As a synergistic integration between spectroscopy and imaging technologies, spectral imaging modalities have been emerged to tackle quality evaluation dilemmas by proposing different designs with effective and practical applications in food and agriculture. With the advantage of acquiring spatio-spectral data across a wide range of the electromagnetic spectrum, the state-of-the-art multispectral imaging in tandem with different multivariate chemometric analysis scenarios has been successfully implemented not only for food quality and safety control purposes, but also in dealing with critical research challenges in seed science and technology. This paper will shed some light on the fundamental configuration of the systems and give a birds-eye view of all recent approaches in the acquisition, processing and reproduction of multispectral images for various applications in seed quality assessment and seed phenotyping issues. This review article continues from where earlier review papers stopped but it only focused on fully-operated multispectral imaging systems for quality assessment of different sorts of seeds. Thence, the review comprehensively highlights research attempts devoted to real implementations of only fully-operated multispectral imaging systems and does not consider those ones that just utilized some key wavelengths extracted from hyperspectral data analyses without building independent multispectral imaging systems. This makes this article the first attempt in briefing all published papers in multispectral imaging applications in seed phenotyping and quality monitoring by providing some examples and research results in characterizing physicochemical quality traits, predicting physiological parameters, detection of defect, pest infestation and seed health.
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                Author and article information

                Journal
                Sensors (Basel)
                Sensors (Basel)
                sensors
                Sensors (Basel, Switzerland)
                MDPI
                1424-8220
                03 August 2020
                August 2020
                : 20
                : 15
                : 4319
                Affiliations
                [1 ]Agronomy Department, Federal University of Viçosa, Viçosa MG 36570-900, Brazil; laercio.silva@ 123456ufv.br (L.J.d.S.); joaop.ribeiro@ 123456ufv.br (J.P.O.R.); abraaoufs@ 123456gmail.com (A.A.S.)
                [2 ]Chemistry Department, Federal University of Viçosa, Viçosa MG 36570-900, Brazil; kamylla.ferreira@ 123456ufv.br
                [3 ]Soil Science Department, University of São Paulo, Piracicaba SP 13418-260, Brazil; jorge.fimrosas@ 123456usp.br
                [4 ]Entomology Department, Federal University of Viçosa, Viçosa MG 36570-900, Brazil
                [5 ]Laboratory of Radiobiology and Environment, University of São Paulo-Center for Nuclear Energy in Agriculture, 303 Centenário Avenue, Piracicaba SP 13416-000, Brazil; clissia_usp@ 123456hotmail.com
                Author notes
                Author information
                https://orcid.org/0000-0002-1097-0292
                https://orcid.org/0000-0001-7202-0420
                https://orcid.org/0000-0002-1163-3546
                https://orcid.org/0000-0002-3244-4816
                https://orcid.org/0000-0001-5284-3294
                https://orcid.org/0000-0002-7279-3260
                Article
                sensors-20-04319
                10.3390/s20154319
                7435829
                32756355
                061ee12e-48f7-473b-9298-7b2cda6d8f89
                © 2020 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 26 June 2020
                : 30 July 2020
                Categories
                Letter

                Biomedical engineering
                germination prediction,linear discriminant analysis,fourier transform near-infrared spectroscopy,radiographic images,urochloa brizantha

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