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      Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg.

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          Abstract

          In this study, antibiotic resistance class 3 integrons in Gram-negative bacteria isolated from hospital sewage and sludge and their genetic contents were characterised. Two samples of hospital effluent from France and Luxembourg and one sample of sludge from a wastewater treatment plant in France were collected in 2010 and 2011. Bacteria were cultured on selective agar plates and integrons were detected in colonies by quantitative PCR. Integron gene cassette arrays and their genetic environments were analysed by next-generation sequencing. Three class 3 integron-positive isolates were detected, including Acinetobacter johnsonii LIM75 (French hospital effluent), Aeromonas allosaccharophila LIM82 (sludge) and Citrobacter freundii LIM86 (Luxembourg hospital effluent). The gene cassettes were all implicated in antibiotic (aminoglycoside and β-lactam) or antiseptic resistance. An oxacillinase gene cassette (blaOXA-10, blaOXA-368 or blaOXA-2) was found in each integron. All of the class 3 integrons were located on small mobilisable plasmids. This study highlights the role of class 3 integrons in the dissemination of clinically relevant antibiotic resistance genes, notably oxacillinase genes, in hospital effluent.

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          Author and article information

          Journal
          Int. J. Antimicrob. Agents
          International journal of antimicrobial agents
          Elsevier BV
          1872-7913
          0924-8579
          Oct 2016
          : 48
          : 4
          Affiliations
          [1 ] INSERM, U1092, F-87000 Limoges, France; Université de Limoges, UMR-S 1092, F-87000 Limoges, France; Centre Pasteur du Cameroun, Yaoundé, Cameroon.
          [2 ] INSERM, U1092, F-87000 Limoges, France; Université de Limoges, UMR-S 1092, F-87000 Limoges, France; Université de Limoges, GRESE, EA 4330, F-87000 Limoges, France.
          [3 ] Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation (ERIN) Department, Belvaux, Luxembourg.
          [4 ] Centre Pasteur du Cameroun, Yaoundé, Cameroon.
          [5 ] INSERM, U1092, F-87000 Limoges, France; Université de Limoges, UMR-S 1092, F-87000 Limoges, France; CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, F-87000 Limoges, France.
          [6 ] INSERM, U1092, F-87000 Limoges, France; Université de Limoges, UMR-S 1092, F-87000 Limoges, France; CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, F-87000 Limoges, France. Electronic address: olivier.barraud@unilim.fr.
          Article
          S0924-8579(16)30187-X
          10.1016/j.ijantimicag.2016.06.018
          27499434
          06d7abff-e55b-421b-81f2-b5e39fb10e0d
          History

          Antimicrobial resistance,Class 3 integron,Effluent,Environment,Oxacillinase

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