Cryptosporidiosis outbreaks linked to the public water supply in a military camp, France

Abstract

Introduction

Contaminated drinking and recreational waters account for most of the reported Cryptosporidium spp. exposures in high-income countries. In June 2017, two successive cryptosporidiosis outbreaks occurred among service members in a military training camp located in Southwest France. Several other gastroenteritis outbreaks were previously reported in this camp, all among trainees in the days following their arrival, without any causative pathogen identification. Epidemiological, microbiological and environmental investigations were carried out to explain theses outbreaks.

Material and methods

Syndromic diagnosis using multiplex PCR was used for stool testing. Water samples (100 L) were collected at 10 points of the drinking water installations and enumeration of Cryptosporidium oocysts performed. The identification of Cryptosporidium species was performed using real-time 18S SSU rRNA PCR and confirmed by GP60 sequencing.

Results

A total of 100 human cases were reported with a global attack rate of 27.8%. Cryptosporidium spp. was identified in 93% of stool samples with syndromic multiplex PCR. The entire drinking water network was contaminated with Cryptosporidium spp. The highest level of contamination was found in groundwater and in the water leaving the treatment plant, with >1,000 oocysts per 100 L. The same Cryptosporidium hominis isolate subtype IbA10G2 was identified in patients’ stool and water samples. Several polluting activities were identified within the protection perimeters of the water resource. An additional ultrafiltration module was installed at the outlet of the water treatment plant. After several weeks, no Cryptosporidium oocysts were found in the public water supply.

Conclusions

After successive and unexplained gastroenteritis outbreaks, this investigation confirmed a waterborne outbreak due to Cryptosporidium hominis subtype IbA10G2. Our study demonstrates the value of syndromic diagnosis for gastroenteritis outbreak investigation. Our results also highlight the importance of better assessing the microbiological risk associated with raw water and the need for sensitive and easy-to-implement tools for parasite detection.

Author summary

Cryptosporidiosis remains a neglected infectious disease, even in high-income countries. Most of the reported cases and outbreaks are related to drinking water and recreational water contaminated with Cryptosporidium spp. In Europe, the search for Cryptosporidium spp. and other parasites in stool or water samples is not routinely performed by laboratories, especially in the absence of dedicated national guidance on testing. In France, cryptosporidiosis is not a notifiable disease. In order to better assess the pathogens involved in foodborne and waterborne disease outbreaks a new outbreak investigation strategy was implemented in the French Armed Forces including: systematic stool sampling, routine syndromic multiplex PCR diagnoses, and pathogens genotyping. After several unexplained gastroenteritis outbreaks in a military camp in France, we identified the same C. hominis IbA10G2 isolate in the stools of patients and in the entire water distribution network. The highest levels of contamination were found in groundwater and in the water leaving the treatment plant. Our study demonstrates the value of syndromic diagnosis for gastroenteritis outbreaks investigation and highlights the importance of better assessing the microbiological risks associated with raw water.