For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
22
views
45
references
 Top references
cited by
3
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      321
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Phytoconstituents from Alpinia purpurata and their in vitro inhibitory activity against Mycobacterium tuberculosis

      other

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Alpinia purpurata or red ginger was studied for its phytochemical constituents as part of our growing interest on Philippine Zingiberaceae plants that may exhibit antimycobacterial activity. The hexane and dichloromethane subextracts of the leaves were fractionated and purified using silica gel chromatography to afford a mixture of C 28–C 32 fatty alcohols, a 3-methoxyflavone and two steroidal glycosides. The two latter metabolites were spectroscopically identified as kumatakenin (1), sitosteryl-3-O-6-palmitoyl-β-D-glucoside (2) and b-sitosteryl galactoside (3) using ultraviolet (UV), infrared (IR), electron impact mass spectrometer (EIMS) and nuclear magnetic resonance (NMR) experiments, and by comparison with literature data. This study demonstrates for the first time the isolation of these constituents from A. purpurata. In addition to the purported anti-inflammatory activity, its phytomedicinal potential to treat tuberculosis is also described.

          Related collections

          Most cited references45

          • Record: found
          • Abstract: found
          • Article: not found

          Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium.

          L. Collins, S Franzblau (1997)
          In response to the need for rapid, inexpensive, high-throughput assays for antimycobacterial drug screening, a microplate-based assay which uses Alamar blue reagent for determination of growth was evaluated. MICs of 30 antimicrobial agents against Mycobacterium tuberculosis H37Rv, M. tuberculosis H37Ra, and Mycobacterium avium were determined in the microplate Alamar blue assay (MABA) with both visual and fluorometric readings and compared to MICs determined in the BACTEC 460 system. For all three mycobacterial strains, there was < or = 1 dilution difference between MABA and BACTEC median MICs in four replicate experiments for 25 to 27 of the 30 antimicrobics. Significant differences between MABA and BACTEC MICs were observed with 0, 2, and 5 of 30 antimicrobial agents against H37Rv, H37Ra, and M. avium, respectively. Overall, MICs determined either visually or fluorometrically in MABA were highly correlated with those determined in the BACTEC 460 system, and visual MABA and fluorometric MABA MICs were highly correlated. MICs of rifampin, rifabutin, minocycline, and clarithromycin were consistently lower for H37Ra compared to H37Rv in all assays but were similar for most other drugs. M. tuberculosis H37Ra may be a suitable surrogate for the more virulent H37Rv strain in primary screening of compounds for antituberculosis activity. MABA is sensitive, rapid, inexpensive, and nonradiometric and offers the potential for screening, with or without analytical instrumentation, large numbers of antimicrobial compounds against slow-growing mycobacteria.
          Article 0 comments Cited 171 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            A randomised placebo-controlled trial of the efficacy of beta-sitosterol and its glucoside as adjuvants in the treatment of pulmonary tuberculosis.

            J Lamprecht, Leah Freestone, C Albrecht … (1997)
            To evaluate the adjuvant effect of beta-sitosterol and its glucoside in the treatment of culture proven pulmonary tuberculosis (PTB). A blinded randomised placebo-controlled trial in culture proven drug sensitive PTB. Patients were hospitalised for the duration of treatment and evaluated at monthly intervals with regard to sputum culture positivity, chest radiography, weight gain, Mantoux test response, routine haematology and liver functions. STATISTICAL EVALUATION: General linear models for repeated measures (SAS GLM package) compared the interaction effects, group effects and time effects of findings in 19 patients receiving sitosterols with those in 18 patients receiving a placebo (talcum powder). Absolute values and change from baseline values were evaluated, although only the latter are reported. Weight gain was significantly greater in the sitosterol group (mean weight gain 8.9 kg) than the placebo group (mean gain 6.1 kg) (P = 0.0023 group effects; P = 0.0001 for time effects). Speed of achieving culture negativity, radiological improvement and induration on Mantoux testing was similar in the two groups. Change in lymphocyte counts from baseline was significantly higher in the sitosterol group (P = 0.0001 and P = 0.0001 for group and time effects) as was the increase in eosinophil counts (P = 0.0001 and P = 0.0137 for group and time effects). The study has shown significantly improved weight gain and higher lymphocyte and eosinophil counts in PTB patients receiving sitosterols in addition to an efficacious antituberculosis regimen. Sitosterols and their possible mode of action should now be evaluated in larger numbers of tuberculosis patients and in diseases with a similar immunopathogenesis.
            Article 0 comments Cited 19 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Antioxidant activity of extracts from Alpinia katsumadai seed.

              T. J. Kim, Hyun-dong Shin, S Lee … (2003)
              Alpinia katsumadai (Zingiberaceae) has been widely used in traditional Chinese medicine to treat a variety of conditions such as emesis and gastric disorders. However, very little is known about the cellular actions by which this plant mediates its therapeutic effects. Various aspects of antioxidant activity were evaluated in a total extract derived from Alpinia katsumadai seed in this study. Relatively high levels of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were detected in the total extract (IC(50) 1.6 microgram/mL). Other known compounds such as (-)-epigallocatechine-3-gallate (EGCG) and resveratrol showed IC(50) values of <0.8 and 4.8 microgram/mL, respectively. The total extract also enhanced the viability of Chinese hamster lung fibroblast (V79-4) cells and inhibited H(2)O(2)-induced apoptosis. The total extract of Alpinia katsumadai also dose-dependently enhanced the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in V79-4 cells, and these effects were comparable to other antioxidant compounds such as EGCG and resveratrol. Taken together, our findings show that Alpinia katsumadai contains significant antioxidant activity. Copyright 2003 John Wiley & Sons, Ltd.
              Article 0 comments Cited 18 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Pharmacogn Mag
                Journal ID (publisher-id): PM
                Title: Pharmacognosy Magazine
                Publisher: Medknow Publications (India )
                ISSN (Print): 0973-1296
                ISSN (Electronic): 0976-4062
                Publication date (Print): Oct-Dec 2010
                Volume: 6
                Issue: 24
                Pages: 339-344
                Affiliations
                Phytochemistry Laboratory, Research Center for the Natural Sciences, Thomas Aquinas Research Complex, University of Santo Tomas, España, Manila 1008, Philippines
                [1 ] University of Paderborn, Department of Chemistry, Warburgerstrasse 100, 33098 Paderborn, Germany
                [2 ] Institute for TB Research, College of Pharmacy, MC 964 Rm 412, University of Illinois at Chicago, 833 S. Wood St., Chicago, IL 60612-7231 USA
                Author notes
                Address for correspondence: Prof. Alicia Aguinaldo, Phytochemistry Laboratory, Research Center for the Natural Sciences, Thomas Aquinas Research Complex, University of Santo Tomas, Espana St., Manila, Philippines, USA. E-mail: alicia.aguinaldo@ 123456gmail.com
                Article
                Publisher ID: PM-6-339
                DOI: 10.4103/0973-1296.71785
                PMC ID: 2992151
                PubMed ID: 21120040
                SO-VID: 105e75f8-78eb-491c-b083-e710d705cd84
                Copyright © © Pharmacognosy Magazine
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 30 March 2010
                Date revision received : 26 July 2010
                Date accepted : 20 October 2010
                Categories
                Subject: Short Communication

                ScienceOpen disciplines: Pharmacology & Pharmaceutical medicine
                Keywords: mycobacterium tuberculosis,kumatakenin,sitosteryl glycosides,alpinia purpurata,fatty alcohols
                Data availability:
                ScienceOpen disciplines: Pharmacology & Pharmaceutical medicine
                Keywords: mycobacterium tuberculosis, kumatakenin, sitosteryl glycosides, alpinia purpurata, fatty alcohols

                Comments

                Comment on this article

                scite_

                Similar content321

                See all similar

                Cited by3

                See all cited by

                Most referenced authors155

                See all reference authors