Corrigendum to “Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the  Mycoplasma capricolum  subsp. Capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine”

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Abstract

In the article titled “Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. Capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine” [1], information was omitted in the Acknowledgments section. The corrected section appears as follows:

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Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine

Jean de Dieu Baziki, Bodjo Charles, Nick Nwankpa … (2020)

An effective contagious caprine pleuropneumonia (CCPP) vaccine is essential for the increased production of healthy goats in a cost-effective manner and the prevention of animal-to-animal transmission for both domestic animals and wildlife. Quality control of this vaccine ensures that a reliable supply of pure, safe, and potent batches is obtained. As part of this control, in vitro quantification of Mycoplasma capricolum subsp. capripneumoniae (Mccp) protein in the final vaccines is required before the CCPP vaccine undergoes batch release and certification. The current method used for quantification is based on the measurement of total protein using the bicinchonic acid (BCA) test. This method quantifies the total amount of protein in the vaccine including contaminant protein from media, which can lead to overestimation of the quantity of Mccp protein, resulting in reduced vaccine immunogenicity. An immuno-capture ELISA (ICE) was developed for specific detection and quantification of the Mccp antigen in the CCPP vaccine. As the ICE detects and measures the amount of antigen between two layers of antibodies, capture and detecting antibodies are required. Mouse monoclonal antibodies (mAbs) that detect the Mccp antigen were produced and characterized. One of these mAbs, Mccp-25, was used to develop the ICE as an unlabelled capture antibody and horseradish peroxidase conjugated detecting antibody. The ICE was standardized and evaluated using an internal reference sample, experimental CCPP vaccines and commercial CCPP vaccines. A comparison between the polymerase chain reaction (PCR) and ICE showed good correlation between the two assays. Also, an in vitro ICE method correlated well with an in vivo sero-conversion in goats that were vaccinated with selected test vaccines. The sensitivity of the ICE was estimated at 30 ng/ml.

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Contributors

Jean de Dieu Baziki:

ORCID: https://orcid.org/0000-0001-7387-9915

Charles Bodjo S.:

ORCID: https://orcid.org/0000-0002-3240-7097

Journal

Journal ID (nlm-ta): Vet Med Int

Journal ID (iso-abbrev): Vet Med Int

Journal ID (publisher-id): vmi

Title: Veterinary Medicine International

Publisher: Hindawi

ISSN (Print): 2090-8113

ISSN (Electronic): 2042-0048

Publication date Collection: 2021

Publication date (Electronic): 23 March 2021

Publication date PMC-release: 23 March 2021

Volume: 2021

Electronic Location Identifier: 9813273

Affiliations

¹African Union-Pan African Veterinary Vaccine Centre (AU-PANVAC), P.O. Box: 1746, Debre Zeit, Ethiopia

²Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), JKUAT Main Campus, P.O. Box: 62000-00200, Nairobi, Kenya

³Jomo Kenyatta University of Agriculture and Technology (JKUAT), P.O. Box: 62000-00200, Nairobi, Kenya

⁴National Veterinary Institute, P.O. Box: 19, Debrezeit, Ethiopia

⁵Addis Ababa University, Department of Microbial Cellular and Molecular Biology, P.O. Box: 1176, Addis Ababa, Ethiopia

⁶Kenya Veterinary Vaccines Production Institute (KEVEVAPI), P.O. Box: 53260-00200, Nairobi, Kenya

Author information

Jean de Dieu Baziki https://orcid.org/0000-0001-7387-9915

Charles Bodjo S. https://orcid.org/0000-0002-3240-7097

Naomi Maina https://orcid.org/0000-0001-7577-5140

Ethel Chitsungo https://orcid.org/0000-0003-0871-5935

Cisse Rahamatou Moustapha Boukary https://orcid.org/0000-0003-0481-2041

Rume Veronica Nwankpa https://orcid.org/0000-0001-9908-8169

Article

DOI: 10.1155/2021/9813273

PMC ID: 8019645

SO-VID: 128d7c7f-47d4-4950-b44d-08cdc555b1bd

License:

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

History

Date received : 17 March 2021

Date accepted : 17 March 2021

Funding

Funded by: The Global Alliance for Livestock Veterinary Medicines

Award ID: AUP-R17A0079

Corrigendum to “Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. Capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine”

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Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine

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