0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Implications of Investigating the Structure-Function Relationship of Human Growth Hormone in Clinical Diagnosis and Therapy

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Human growth hormone (GH) mediates longitudinal bone growth and also exerts a variety of other biological effects, e.g. lactogenic, insulin-like, diabetogenic, lipolytic, protein-anabolic and sodium/water-retaining effects. Investigation of the structure-function relationship of human GH has been attempted by epitope mapping using monoclonal antibodies and by systematic point mutations of the human GH molecule. The diagnosis of GH-related disorders is complicated by the fact that different commercial immunoassay kits give widely differing results. This phenomenon cannot be explained by lack of standardization, but has to be attributed to the epitope specificities of the antibodies employed in the assay techniques and the spectra of different molecular forms of human GH recognized by them. The information required by a clinician in ordering a GH determination is the bioactivity of all the human GH forms in the sample, rather than the immunoreactivity with a given set of antibodies. Therefore, the aim of future GH immunoassays must be the identification of antibodies that bind with high affinity only those forms of human GH that bind and activate the GH receptor. Epitope mapping by monoclonal antibodies appears to be the ideal approach to this goal. It has recently been shown that one molecule of human GH binds two molecules of GH receptor, and dimerization of the receptor by its ligand is a prerequisite for biological function. Improvements in understanding the structure-function relationship of the human GH molecule and of the interplay of the hormone with its receptor make it conceivable that recombinant analogues of human GH will be designed to inhibit the effects of GH excess in acromegaly. Furthermore, analogues of human GH could be designed to exhibit a modified spectrum of biological activities compared with wild-type human GH.

          Related collections

          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          978-3-8055-6003-0
          978-3-318-00608-7
          1663-2818
          1663-2826
          1994
          1994
          05 December 2008
          : 41
          : Suppl 2
          : 113-120
          Affiliations
          Medizinische Klinik, Klinikum Innenstadt der Ludwig- Maximilians-Universität München, FRG
          Article
          183970 Horm Res 1994;41:113–120
          10.1159/000183970
          7522207
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 8
          Categories
          Symposium Session II: Hormone Receptor Physiology and Novel Therapeutic Approaches

          Comments

          Comment on this article