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      Retinal Pigment Epithelium Cells Promote the Maturation of Monocytes to Macrophages in vitro

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          Abstract

          Proliferative vitreoretinopathy is characterized by excessive cell proliferation within the eye; retinal pigment epithelial (RPE) cells form the majority of proliferating cells and interact with infiltrating leukocytes including monocytes. The purpose of this study was to determine the effect of RPE cells on the maturation of monocytes to macrophages. The enriched monocyte fraction of peripheral blood mononuclear cells was either cultured with or without RPE cells. The expression of the maturation-associated antigen CD 16 on monocytes was assessed by flow cytometry, and the concentration of bioactive transforming growth factor-β (TGF-β) in the culture supernatant measured by mink lung epithelial cell (Mv1Lu) bioassay. The cellular density of CD 16 in terms of mean fluorescence intensity was significantly higher on monocytes in coculture with RPE cells (p = 0.0153) than on monocytes in monoculture. The CD 16 expression was significantly (p = 0.0093) reduced when antibodies to TGF-β were added to the culture medium. RPE cells did not express CD 16. Supernatants from cocultures also contained active TGF-β (76.7 ± 23.8 pg/ml), while in those of cell monocultures TGF-β was close to the detection limit. We conclude that RPE cells stimulate and modulate the differentiation of monocytes to macrophages. Bioactive TGF-β generated in the coculture was in part responsible for this effect. It seems likely that RPE cells or interactions between RPE cells and monocytes could be an important factor in inflammatory/immune processes and wound healing in the eye, which are probably involved in proliferative vitreoretinopathy.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1997
          1997
          11 December 2009
          : 29
          : 1
          : 31-36
          Affiliations
          aDepartment of Ophthalmology, University of Southern California School of Medicine and the Doheny Eye Institute, and bDivision of Research Immunology and Bone Marrow Transplantation, Childrens Hospital of Los Angeles, Los Angeles, Calif., USA; cUniversity Eye Hospital, Basel, Switzerland
          Article
          267988 Ophthalmic Res 1997;29:31–36
          10.1159/000267988
          9112264
          1b44e382-89a0-4d35-bbdc-088623a43f44
          © 1997 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 01 February 1996
          : 26 September 1996
          Page count
          Pages: 6
          Categories
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Retinal pigment epithelial cells,Coculture,Monocyte differentiation CD16,Transforming growth factor-β

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