Bacterial laccases: promising biological green tools for industrial applications

Pretreatment is an important step involved in the production of bioethanol from lignocelluosic biomass. Though several pretreatment regimes are available, biological pretreatment seems to be promising being an eco-friendly process and there is no inhibitor generation during the process. In the current scenario there are few limitations in using this strategy for pilot scale process. The first and foremost one is the long incubation time for effective delignification. This can be minimized to an extent by using suitable microbial consortium. There is an urgent need for research and development activities and fine tuning of the process for the development of an economically viable process. This review presents an overview of various aspects of biological pretreatment, enzymes involved in the process, parameters affecting biological pretreatment as well as future perspectives.

Laccases are oxidoreductases which oxidize a variety of aromatic compounds using oxygen as the electron acceptor and producing water as by-product. The interest for these old enzymes (first described in 19th century) has progressively increased due to their outstanding biotechnological applicability. The presence of redox mediators is required for a number of biotechnological applications, providing the oxidation of complex substrates not oxidized by the enzyme alone. The efficiency of laccase-mediator systems to degrade recalcitrant compounds has been demonstrated, but still the high cost and possible toxicity of artificial mediators hamper their application at the industrial scale. Here, we present a general outlook of how alternative mediators can change this tendency. We focus on phenolic compounds related to lignin polymer that promotes the in vitro transformation of recalcitrant non-phenolic structures by laccase and are seemingly the natural mediators of laccases. The use of eco-friendly mediators easily available from lignocellulose, could contribute to the industrial implementation of laccases and the development of the 21th century biorefineries. Copyright © 2010 Elsevier Inc. All rights reserved.