Characteristics of Hormone-Stimulated Adenylate Cyclase in Vascular Smooth Muscle: Altered Activity in Spontaneously Hypertensive Rat

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

The stimulation of adenylate cyclase activity by guanine nucleotides, isoproterenol and fluoride has been investigated in KC1 washed rat aortic membranes. We observed that the presence of guanine nucleotides in the assay was obligatory for the isoproterenol stimulation of adenylate cyclase activity. Guanine nucleotides could stimulate adenylate cyclase in the absence of catecholamines; however, the extent of stimulation was significantly lower (p < 0.05) than that observed with isoproterenol. The order of effectiveness of guanine nucleotides was guanosine 5’-0-(3-thiotriphosphate) (GTP-γ-S), 5’-guanylylimido diphosphate (Gpp(NH)p), and guanosine 5’-triphosphate (GTP). GTP stimulated enzyme activity without a noticeable lag period, while stimulation by Gpp(NH)p showed a lag period of 3–4 min. Addition of isoproterenol neither abolished the lag period, nor altered the apparent K<sub>a</sub> value (concentration required for half maximal stimulation) for Gpp(NH)p stimulation. In a comparative study of adenylate cyclase activity between spontaneously hypertensive rats (SHR) and Kyoto Wistar normotensive rats (WKY), using 0.6 M KC1 washed membranes from aorta and caudal artery, no differences were observed in the basal adenylate cyclase activity. However, guanine nucleotide-, isoproterenol-, and fluoride-stimulated enzyme activity was significantly lower (p < 0.05) in the caudal artery of SHR as compared to WKY.

Related collections

Author and article information

Journal

Journal ID (publisher-id): JVR

Journal ID (nlm-ta): J Vasc Res

Journal ID (doi): 10.1159/issn.1018-1172

Title: Journal of Vascular Research

Publisher: S. Karger AG

ISSN (Print): 1018-1172

ISSN (Electronic): 1423-0135

Publication date Subscription-year: 1982

Publication date (Print): 1982

Publication date (Electronic): 19 September 2008

Volume: 19

Issue: 3

Pages: 109-116

Affiliations

Department of Anatomy and Cardiovascular Center, University of Iowa, Medical Center, Iowa City, Iowa, USA

Article

Publisher ID: 158377 Other ID: Blood Vessels 1982;19:109–116

DOI: 10.1159/000158377

SO-VID: 1d182695-d0df-46fc-af82-4b57ee430966

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 04 April 1981

Date accepted : 10 July 1981

Page count

Pages: 8

Comments

Comment on this article

scite_

Call for Papers: Novel Methods in Vascular and Lymphatic Physiology

Submit here before June 30, 2025