Utility of Histologic and Histochemical Screening for 16S Ribosomal RNA Gene Sequencing
      of Formalin-Fixed, Paraffin-Embedded Tissue for Bacterial Endocarditis.

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Abstract

16S ribosomal RNA (rRNA) sequencing is a powerful but expensive tool for the identification of bacteria in culture-negative endocarditis. Histologic criteria to screen formalin-fixed, paraffin-embedded (FFPE) specimens for testing are evaluated.

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Journal

Journal ID (iso-abbrev): Am J Clin Pathol

Title: American journal of clinical pathology

Publisher: Oxford University Press (OUP)

ISSN (Electronic): 1943-7722

ISSN (Print): 0002-9173

Publication date (Electronic): September 09 2019

Volume: 152

Issue: 4

Affiliations

[1 ] Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.

Article

Publisher Item ID: 5512258

DOI: 10.1093/ajcp/aqz055

PubMed ID: 31172185

SO-VID: 1f81b9de-e31c-4117-b1d5-4e6579e669a3

History

Keywords: paraffin-embedded tissue,Warthin-Starry stain,Molecular diagnostics,Histologic features,Grocott methenamine silver stain,Gram stain,Formalin-fixed,Bacterial endocarditis,16S rRNA gene sequencing

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Keywords: paraffin-embedded tissue, Warthin-Starry stain, Molecular diagnostics, Histologic features, Grocott methenamine silver stain, Gram stain, Formalin-fixed, Bacterial endocarditis, 16S rRNA gene sequencing

Utility of Histologic and Histochemical Screening for 16S Ribosomal RNA Gene Sequencing of Formalin-Fixed, Paraffin-Embedded Tissue for Bacterial Endocarditis.

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RNA drug delivery

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