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Abstract
Three unique NADPH:cytochrome P450 reductase (CPR) cDNAs have been isolated from a
Nothapodytes foetida cDNA library and characterized. Phylogenetic analysis showed
that NfCPR1 is a class I isoform, whereas NfCPR2 and NfCPR3 are class II isoforms.
Both NfCPR1 and NfCPR2 transcripts were detected in all examined organs of N. foetida,
with the highest level for NfCPR1 being in the seeds whereas for NfCPR2 predominantly
in leaves. In contrast, NfCPR3 transcripts were only detected in flower buds and seeds
at almost equal expression levels. Moreover, NfCPR1 expression did not change during
wounding treatment, whereas NfCPR2 and NfCPR3 were induced in response to wounding.
Microsomes isolated from insect cells co-expressing NfCPR2 and cytochrome P450 enzyme
geraniol 10-hydroxylase (G10H) enhanced the production of eriodictyol from naringenin
approximately 11-fold relative to control G10H-only insect cells, indicating the supportive
role of NfCPR2 for G10H activity in insect cells.