Accordion-Like Honeycombs for Tissue Engineering of Cardiac Anisotropy

Abstract

Tissue engineered grafts may be useful in myocardial repair, however previous scaffolds have been structurally incompatible with recapitulating cardiac anisotropy. Utilizing microfabrication techniques, a novel accordion-like honeycomb microstructure was rendered in poly(glycerol sebacate) to yield porous, elastomeric 3-D scaffolds with controllable stiffness and anisotropy. Accordion-like honeycomb scaffolds with cultured neonatal rat heart cells demonstrated utility via: (1) closely matched mechanical properties compared to native adult rat right ventricular myocardium, with stiffnesses controlled by polymer curing time; (2) heart cell contractility inducible by electric field stimulation with directionally-dependent electrical excitation thresholds (p<0.05); and (3) greater heart cell alignment (p<0.0001) than isotropic control scaffolds. Prototype bilaminar scaffolds with 3-D interconnected pore networks yielded electrically excitable grafts with multi-layered neonatal rat heart cells. Accordion-like honeycombs can thus overcome principal structural-mechanical limitations of previous scaffolds, promoting the formation of grafts with aligned heart cells and mechanical properties more closely resembling native myocardium.