Development and validation of a UPLC–MS/MS method for the simultaneous determination of paritaprevir and ritonavir in rat liver

Abstract

Aim:

Determination of paritaprevir and ritonavir in rat liver tissue samples.

Results:

We successfully validated a UPLC–MS/MS method to measure paritaprevir and ritonavir in rat liver using deuterated internal standards ( d8-paritapervir and d6-ritonavir). The method is linear from 20 to 20,000 and 5 to 10,000 pg on column for paritaprevir and ritonavir, respectively, and is normalized per milligram tissue. Interday and intraday variability ranged from 0.591 to 5.33% and accuracy ranged from -6.68 to 10.1% for quality control samples. The method was then applied to the measurement of paritaprevir and ritonavir in rat liver tissue samples from a pilot study.

Conclusion:

The validated method is suitable for measurement of paritaprevir and ritonavir within rat liver tissue samples for PK studies.