The coronavirus avian infectious bronchitis virus requires the cell nucleus and host transcriptional factors

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Abstract

Replication of avian infectious bronchitis virus in permissive BHK-21 cells is blocked when these cells are enucleated or irradiated with ultraviolet light prior to infection, or if cells are treated with α-amanitin during the virus growth cycle. This coronavirus, like influenza virus, can replicate normally in the presence of α-amanitin in Chinese hamster ovary cells which possess a drug-resistant RNA polymerase II. These findings indicate that avian infectious bronchitis virus requires the intact cell nucleus and one or more host transcriptional functions for productive infections. Preliminary data suggest that these cellular functions involve some aspect of virus-directed RNA synthesis.

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Author and article information

Journal

Journal ID (nlm-ta): Virology

Journal ID (iso-abbrev): Virology

Title: Virology

Publisher: Published by Elsevier Inc.

ISSN (Print): 0042-6822

ISSN (Electronic): 1096-0341

Publication date PMC-release: 6 February 2004

Publication date (Print): September 1980

Publication date (Electronic): 6 February 2004

Volume: 105

Issue: 2

Pages: 582-591

Affiliations

Waksman Institute of Microbiology, Rutgers—The State University, P.O. Box 759, Piscataway, New Jersey 08854 USA

Author notes

[1 ]To whom reprint requests should be addressed.

Article

Publisher ID: 0042-6822(80)90058-6

DOI: 10.1016/0042-6822(80)90058-6

PMC ID: 7130657

PubMed ID: 6158788

SO-VID: 2609ef8e-b99c-4794-b7cb-798cb47ceb8c

License:

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