Interleaflet coupling of n-alkane incorporated bilayers

Author(s): Hatsuho Usuda ¹ ^, ² ^, ³ ^, ⁴ ^, ⁵ , Mafumi Hishida ¹ ^, ² ^, ³ ^, ⁴ ^, ⁵ , Elizabeth G. Kelley ⁶ ^, ⁷ ^, ⁸ ^, ⁹ , Yasuhisa Yamamura ¹ ^, ² ^, ³ ^, ⁴ ^, ⁵ , Michihiro Nagao ⁶ ^, ⁷ ^, ⁸ ^, ⁹ ^, ¹⁰ , Kazuya Saito ¹ ^, ² ^, ³ ^, ⁴ ^, ⁵

Publication date (Electronic): 2020

Journal: Physical Chemistry Chemical Physics

Publisher: Royal Society of Chemistry (RSC)

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Abstract

The relationship between the membrane bending modulus ( κ) and compressibility modulus ( K _A) depends on the extent of coupling between the two monolayers (leaflets).

Abstract

The relationship between the membrane bending modulus ( κ) and compressibility modulus ( K _A) depends on the extent of coupling between the two monolayers (leaflets). Using neutron spin echo (NSE) spectroscopy, we investigate the effects of n-alkanes on the interleaflet coupling of 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC) bilayers. Structural studies with small-angle X-ray and neutron scattering (SAXS and SANS) showed that the bilayer thickness increased with increasing n-alkane length, while NSE suggested that the bilayers became softer. Additional measurements of the membrane thickness fluctuations with NSE suggested that the changes in elastic moduli were due to a decrease in coupling between the leaflets upon addition of the longer n-alkanes. The decreased coupling with elongating n-alkane length was explained based on the n-alkane distribution within the bilayers characterized by SANS measurement of bilayers composed of protiated DPPC and deuterated n-alkanes. A higher fraction of the incorporated long n-alkanes were concentrated at the central plane of the bilayers and decreased the physical interaction between the leaflets. Using NSE and SANS, we successfully correlated changes in the mesoscopic collective dynamics and microscopic membrane structure upon incorporation of n-alkanes.