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      New Developments in Kidney Development


      S. Karger AG

      Allograft, Metanephros, Transplantation

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          Background/Aims: The number of kidney transplantations performed per year is limited due to the availability of donor organs. One possible solution to the organ shortage is the use of renal xenografts. However, the transplantation of xenografts is complicated by rejection. Methods: It has been postulated that the host immune response might be attenuated following the transplantation of embryonic kidneys (metanephroi) rather than developed (adult kidneys). Transplanted metanephroi become chimeric organs in that their blood supply originates from the host. It is possible to transplant a developing metanephros, without the use of immunosuppression, from one outbred rat to another. Results: Transplanted metanephroi grow, develop, become vascularized, and function in host rats. In contrast, developed adult kidneys transplanted from one rat to another undergo rejection within 7 days after transplantation. Conclusions: These observations suggest that metanephric tissue may be less immunogenic than adult kidney. Transplantation of metanephroi represents a new development that could lead to a novel therapeutic approach to the treatment of chronic renal failure.

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          A simple procedure for the isolation of rat kidney lysosomes.

          A procedure for the isolation of highly purified lysosomes from normal rat kidney is described. The method depends on the swelling of mitochondria when the postnuclear supernatant fraction is incubated with 2 mM Ca2+. The lysosomes can then be separated from the swollen mitochondria by Percoll density gradient centrifugation. The lysosomal fraction obtained by our method was enriched more than 30-fold in terms of marker enzymes with a yield of about 11%. Electron microscopic examination and the measurement of the activities of marker enzymes for various subcellular organelles indicated that our lysosomal preparation was essentially free from contamination by other organelles. We believe that this procedure for isolating kidney lysosome will be useful in the study of the mechanisms of specific modification, processing and catabolism of proteins.

            Author and article information

            S. Karger AG
            10 February 1999
            : 81
            : 2
            : 131-135
            George M. O’Brien Kidney and Urological Disease Center, Renal Division, Departments of Medicine, Pathology, and Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Mo., USA
            45267 Nephron 1999;81:131–135
            © 1999 S. Karger AG, Basel

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            Page count
            Figures: 3, References: 17, Pages: 5
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            Distinguished Scientists Lecture Series Section Editors: J.C.M. Chan; R.J. Krieg, Jr.; J.I. Scheinmann, Richmond, Va.

            Cardiovascular Medicine, Nephrology

            Transplantation, Metanephros, Allograft


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