Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition.

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Abstract

The identification of new biomarkers for early detection of highly recurrent head and neck cancer is urgently needed. MicroRNAs (miRNAs) are small and non-coding RNAs that regulate cancer-related gene expression, such as tumor protein 53 (TP53) gene expression. This study was carried out to analyze TP53 gene expression using real-time PCR and to determine changes in intracellular p53 level by flow cytometry after downregulation of miRNA-181a miRNA inhibitor in the FaDu cell line. TP53 gene expression showed a 3-fold increment and the p53 protein level was also increased in the miRNA-181a-treated cells. In conclusion, miRNA-181a binds to the TP53 gene and inhibits its expression, decreasing the synthesis of p53.

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Journal

Journal ID (iso-abbrev): Genet. Mol. Res.

Title: Genetics and molecular research : GMR

Publisher: Genetics and Molecular Research

ISSN (Electronic): 1676-5680

ISSN (Print): 1676-5680

Publication date (Electronic): Mar 17 2014

Volume: 13

Issue: 1

Affiliations

[1 ] Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia ykcheah@medic.upm.edu.my.

[2 ] Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.

[3 ] Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia.

[4 ] Biomarketing Services (M) Sdn. Bhd., Bandar Menjalara, Kuala Lumpur, Malaysia.

Article

Publisher Item ID: gmr3207

DOI: 10.4238/2014.January.22.4

PubMed ID: 24535903

SO-VID: 2b15e5b4-12ff-44b4-a69e-7d6c606a4ab6

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