It has been 25 years since the publication of the first checklist of mosquito in Bangladesh and several significant taxonomic changes have occurred. Therefore, considering these changes, we prepare an updated list of Anopheles species in Bangladesh along with their distribution maps. A total of 36 Anopheles species have been listed from Bangladesh and these species belong to either the sub-genus Anopheles or Cellia but we captured 30 species in our study. Eleven species were distributed all over Bangladesh but Anopheles nivipes and An. turkhudi were found only in Bandarban and Cox’s Bazar areas respectively. Anopheles sundaicus were found limited number in the costal belt of Bangladesh. Nearly all Anopheles species were found in Southeastern hilly parts of Bangladesh. However, An. vagus, An. philippinensis, An. barbirostris distribute all over the Bangladesh with high density.