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Abstract
A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion
phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal
antibody against CL was labeled with UCP beads. The detection strategy is based on
competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or
CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one
single test without complicated sample preparation. Sensing results can be obtained
within 10 min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA
for CL was 0.1 ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01 ng/mL, could be
achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged
from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay
was evaluated with spiked and real samples and the results were compared with liquid
chromatography-tandem mass. The developed novel assay method based on UCP could be
a potential alternative format for on site and rapid detection of CL as well as other
illegal drugs.