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Isolation of adipose-derived stem cells by using a subfractionation culturing method.
TacGhee Yi
1 ,
Wang-Kyun Kim ,
Joon-Seok Choi ,
Seung Yong Song ,
Juhee Han ,
Ji Hye Kim ,
Won-Serk Kim ,
Sang Gyu Park ,
Hyun-Joo Lee ,
Yun Kyoung Cho ,
Sung-Joo Hwang ,
Sun U Song ,
Jong-Hyuk Sung
Nov 2014
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Abstract
Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps.