Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B1 in Rice

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Abstract

An electrochemical immunosensor for detection of aflatoxin B1 (AFB1) in food samples was developed. The sensor is composed by cysteine monolayer immobilized on gold electrode surface with subsequent bind to monoclonal antibody (MAb-Cys-modified electrode). The AFB1 detection was evaluated by cyclic voltammetry and impedance spectroscopy in the frequency range of 100 mHz-100 kHz. Samples of rice were spiked with AFB1 to evaluate the sensitivity of the sensor. Impedance spectra could be fitted to a Randles equivalent circuit containing a constant phase element. Atomic force microscope (AFM) images showed MAb-AFB1 complex immobilized across the electrode surface. The electron transfer resistance (Rct) increase was attributed to a decrease in the charge permeability of the MAb-AFB1-Au surface to a redox probe K4[Fe(CN)6]4-/K3[Fe(CN)6]3-. The surface coverage exhibited a linear relationship as function of toxin concentration and is found to be 0.75 at 30 µg mL-1. The obtained sensor is a promising candidate for detection of AFB1 in rice with sensitivity and specificity.

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Electrochemical Methods : Fundamentals and Applications

Allen J. Bard, Larry R. Faulkner (2000)

This edition is fully revised to reflect the current state off the field.<br>* Significant additions include ultramicroelectrodes, modified electrodes, and scanning probe methods.<br>* Many chapters have been modified and improved, including electrode kinetics, voltammetric methods, and mechanisms of coupled chemical reactions.

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Novel and highly sensitive fluorescent assay for leucine aminopeptidases.

Huazhang Huang, Hiromasa Tanaka, Bruce Hammock … (2009)

l-Leucine aminopeptidases (LAPs) are implicated in the progress of many pathological disorders and play some regulatory roles in tumor cell proliferation, invasion, and/or angiogenesis. Thus, LAPs not only could become new diagnostic or prognostic biomarkers but also may have potential as novel molecular targets for the treatment of several cancers. Highly sensitive assays are critical for early detection of changes in LAP activity and for screening potent LAP inhibitors. In this study, we developed a novel and highly sensitive fluorescent assay for LAPs based on substituted aminopyridines as fluorescent reporters. This assay was at least 100- and 20-fold more sensitive than commercial colorimetric and fluorescent LAP substrates, respectively. We also showed that this assay was a useful tool for monitoring LAP activities in extracts from cancer cell lines, as well as for the high-throughput screening of inhibitors, which could lead to new cancer treatments.