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      Computer Methods for Automatic Locomotion and Gesture Tracking in Mice and Small Animals for Neuroscience Applications: A Survey

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          Abstract

          Neuroscience has traditionally relied on manually observing laboratory animals in controlled environments. Researchers usually record animals behaving freely or in a restrained manner and then annotate the data manually. The manual annotation is not desirable for three reasons; (i) it is time-consuming, (ii) it is prone to human errors, and (iii) no two human annotators will 100% agree on annotation, therefore, it is not reproducible. Consequently, automated annotation for such data has gained traction because it is efficient and replicable. Usually, the automatic annotation of neuroscience data relies on computer vision and machine learning techniques. In this article, we have covered most of the approaches taken by researchers for locomotion and gesture tracking of specific laboratory animals, i.e. rodents. We have divided these papers into categories based upon the hardware they use and the software approach they take. We have also summarized their strengths and weaknesses.

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          Most cited references135

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          You Only Look Once: unified, real-time object detection

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            Object tracking

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              The use of a plus-maze to measure anxiety in the mouse.

              R Lister (1987)
              To investigate whether an elevated plus-maze consisting of two open and two closed arms could be used as a model of anxiety in the mouse, NIH Swiss mice were tested in the apparatus immediately after a holeboard test. Factor analysis of data from undrugged animals tested in the holeboard and plus-maze yielded three orthogonal factors interpreted as assessing anxiety, directed exploration and locomotion. Anxiolytic drugs (chlordiazepoxide, sodium pentobarbital and ethanol) increased the proportion of time spent on the open arms, and anxiogenic drugs (FG 7142, caffeine and picrotoxin) reduced this measure. Amphetamine and imipramine failed to alter the indices of anxiety. The anxiolytic effect of chlordiazepoxide was reduced in mice that had previously experienced the plus-maze in an undrugged state. Testing animals in the holeboard immediately before the plus-maze test significantly elevated both the percentage of time spent on the open arms and the total number of arm entries, but did not affect the behavioral response to chlordiazepoxide. The plus-maze appears to be a useful test with which to investigate both anxiolytic and anxiogenic agents.
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                Author and article information

                Journal
                Sensors (Basel)
                Sensors (Basel)
                sensors
                Sensors (Basel, Switzerland)
                MDPI
                1424-8220
                25 July 2019
                August 2019
                : 19
                : 15
                : 3274
                Affiliations
                Multimedia and Telecommunications Department, Universitat Oberta de Catalunya, 08018 Barcelona, Spain
                Author notes
                [* ]Correspondence: abbas@ 123456uoc.edu
                [†]

                These authors contributed equally to this work.

                Author information
                https://orcid.org/0000-0002-3544-1549
                https://orcid.org/0000-0001-7898-1847
                Article
                sensors-19-03274
                10.3390/s19153274
                6696321
                31349617
                367bc3f4-1129-4f27-965e-1d552f5993bc
                © 2019 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 31 May 2019
                : 21 July 2019
                Categories
                Review

                Biomedical engineering
                locomotion tracking,gesture tracking,behavioral phenotyping,automated annotation,neuroscience,machine learning

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