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      Reconstructing breastfeeding and weaning practices in the Bronze Age Near East using stable nitrogen isotopes

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          Abstract

          Objectives

          Breastfeeding and childhood diet have significant impact on morbidity and mortality within a population, and in the ancient Near East, it is possible to compare bioarchaeological reconstruction of breastfeeding and weaning practices with the scant textual evidence.

          Materials and Methods

          Nitrogen stable isotopes (δ 15N) are analyzed here for dietary reconstruction in skeletal collections from five Bronze Age (ca. 2,800–1,200 BCE) sites in modern Lebanon and Syria. We employed Bayesian computational modeling on cross‐sectional stable isotope data of collagen samples ( n = 176) mainly from previous studies to test whether the bioarchaeological evidence aligns with the textual evidence of breastfeeding and weaning practices in the region, as well as compare the estimated weaning times to the global findings using the WARN (weaning age reconstruction with nitrogen isotope analysis) Bayesian model.

          Results

          Though the Near East sites in this study had different ecological settings and economic strategies, we found that weaning was introduced to the five sites at 0.5 ± 0.2 years of age and complete weaning occurred around 2.6 ± 0.3 years of age on using the WARN computational model. These weaning processes are within the time suggested by historical texts, though average estimated weaning age on the Mediterranean coast is later than inland sites.

          Discussion

          Compared globally, these Near Eastern populations initiated the weaning process earlier but completed weaning within the global average. Early initial weaning may have created short spacing between pregnancies and a high impact on demographic growth within these agricultural populations, with some variation in subsistence practices accounting for the inland/coastal discrepancies.

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          Most cited references72

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          Approximate Bayesian Computation in Evolution and Ecology

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            Improved Collagen Extraction by Modified Longin Method

            A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.
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              Collagen turnover in the adult femoral mid-shaft: modeled from anthropogenic radiocarbon tracer measurements.

              We have measured the (14)C content of human femoral mid-shaft collagen to determine the dynamics of adult collagen turnover, using the sudden doubling and subsequent slow relaxation of global atmospheric (14)C content due to nuclear bomb testing in the 1960s and 1970s as a tracer. (14)C measurements were made on bone collagen from 67 individuals of both sexes who died in Australia in 1990-1993, spanning a range of ages at death from 40 to 97, and these measurements were compared with values predicted by an age-dependent turnover model. We found that the dataset could constrain models of collagen turnover, with the following outcomes: 1) Collagen turnover rate of females decreases, on average, from 4%/yr to 3%/yr from 20 to 80 years. Male collagen turnover rates average 1.5-3%/yr over the same period. 2) For both sexes the collagen turnover rate during adolescent growth is much higher (5-15%/yr at age 10-15 years), with males having a significantly higher turnover rate than have females, by up to a factor of 2. 3) Much of the variation in residual bomb (14)C in a person's bone can be attributed to individual variation in turnover rate, but of no more than about 30% of the average values for adults. 4) Human femoral bone collagen isotopically reflects an individual's diet over a much longer period of time than 10 years, including a substantial portion of collagen synthesised during adolescence.
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                Author and article information

                Contributors
                chris.stantis@gmail.com
                Journal
                Am J Phys Anthropol
                Am. J. Phys. Anthropol
                10.1002/(ISSN)1096-8644
                AJPA
                American Journal of Physical Anthropology
                John Wiley & Sons, Inc. (Hoboken, USA )
                0002-9483
                1096-8644
                02 December 2019
                May 2020
                : 172
                : 1 ( doiID: 10.1002/ajpa.v172.1 )
                : 58-69
                Affiliations
                [ 1 ] Faculty of Science and Technology, Department of Archaeology and Anthropology Bournemouth University Dorset UK
                [ 2 ] Department of Bioarchaeology Institute of Archaeology, University of Warsaw Warszawa Poland
                Author notes
                [*] [* ] Correspondence

                Chris Stantis, Faculty of Science and Technology, Department of Archaeology and Anthropology, Bournemouth University, Fern Barrow, Poole, Dorset BH12 5BB, UK.

                Email: chris.stantis@ 123456gmail.com

                Author information
                https://orcid.org/0000-0001-7645-0233
                https://orcid.org/0000-0002-9040-5022
                Article
                AJPA23980
                10.1002/ajpa.23980
                7217027
                31797366
                38984ab6-8456-4d1e-bffe-ed29fe405955
                © 2019 The Authors. American Journal of Physical Anthropology published by Wiley Periodicals, Inc.

                This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

                History
                : 24 April 2019
                : 13 August 2019
                : 13 November 2019
                Page count
                Figures: 5, Tables: 6, Pages: 12, Words: 10006
                Funding
                Funded by: Narodowe Centrum Nauki , open-funder-registry 10.13039/501100004281;
                Award ID: 2012/06/M/HS3/00272
                Categories
                Research Article
                Research Articles
                Custom metadata
                2.0
                May 2020
                Converter:WILEY_ML3GV2_TO_JATSPMC version:5.8.1 mode:remove_FC converted:12.05.2020

                Anthropology
                bayesian analysis,childhood,diet,infancy,lebanon,stable isotope analysis,syria
                Anthropology
                bayesian analysis, childhood, diet, infancy, lebanon, stable isotope analysis, syria

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