The present study aimed to investigate the effects of Cuminum cyminum L. essential oil (CEO) and its nanoemulsion (CEON) on oxidative stability and microbial growth of mayonnaise during storage. The GC analysis indicated that Cuminaldehyde (27.99%), o‐Cymene (17.31%), γ‐Terpinen (16.67%), and β‐Pinene (9.35%) were the major components of CEO, respectively. The assessments of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) showed that Escherichia coli ATCC 25922 (MBC CEO = 12 and MBC CEON = 6 mg/mL) was the most resistant bacteria, and in contrast, Staphylococcus aureus ATCC 29213 (MBC CEO = 6 and MBC CEON = 3 mg/mL) was the most sensitive bacteria. In the radical‐scavenging assay, CEON (IC 50 = 5 ± 0.07 μg/mL) exhibited a higher antioxidant activity than CEO (IC 50 = 10 ± 0.13 μg/mL). The results showed that applying the MBC of CEO and CEON in mayonnaise led to a significant decrease ( p < .05) in acidity, peroxide value, number of acid‐resistant bacteria and fungi, and total microbial count compared with the control sample. In conclusion, this study demonstrated that using CEON resulted in oxidative stability, microbial growth control, and desirable sensorial attributes in mayonnaise compared with CEO and control samples.
Nowadays, there is a strong tendency to use natural preservatives such as essential oils in food products. Cuminum cyminum L. essential oil nanoemulsion (CEON) has considerable antioxidant and antimicrobial activities. CEON can more effectively result in the oxidative stability of mayonnaise compared with pure essential oil. CEON can more effectively control the microbial growth of mayonnaise compared with pure essential oil.