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      Regulation of Apolipoprotein A-1 and E Gene Expression in Liver and Intestine of Nephrotic and Pair-Fed Rats

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          Rats treated with puromycin aminonucleoside (PAN) developed characteristics of the nephrotic syndrome, including albuminuria, hypoalbuminemia and hyperiipidemia. To study the regulation of apolipoprotein (apo) A-1 and apo E gene expression in nephrotic rats, we analyzed the steady-state levels (SSLs) of hepatic and intestinal apo A-1 and apo E mRNA using the Northern technique, and the plasma levels of high-density lipoprotein (HDL) by biochemical methods. Male Wistar rats were treated with PAN and compared with pair-fed and untreated control rats at different stages of disease. Nephrotic rats presented with marked hypoalbuminemia and albuminuria at between 6 and 11 days after PAN treatment. During this stage of disease, plasma levels of HDL were elevated in correlation with an increase of both hepatic and intestinal apo A-1 mRNA. In liver of nephrotic rats, high levels of apo A-1 mRNA together with low levels of apo E mRNA caused an increase in the ratio of apo A-1/apo E mRNA, reaching a maximum 6 days after treatment. Apo E mRNA was barely detected in small intestine of pair-fed controls and PAN-treated rats. However, contrary to nephrotic rats, the ratio apo A-1/apo E mRNA was inverted in liver of pair-fed rats due to an increase in apo E mRNA. In conclusion, in nephrotic rats, the SSL of apo A-1 mRNA is increased in liver and small intestine and appears to regulate the plasma levels of apo A-1. These results also suggest a coordinated regulation of the apo A-1 and apo E gene expression in liver of nephrotic and pair-fed rats.

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          Author and article information

          S. Karger AG
          12 December 2008
          : 65
          : 1
          : 100-107
          aMolecular Hepatology Unit, Department of Gastroenterology, and bDepartment of Nephrology and Mineral Metabolism, Instituto Nacional de la Nutrición Salvador Zubirán, Tlalpan, México D.F., México
          187449 Nephron 1993;65:100–107
          © 1993 S. Karger AG, Basel

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          Page count
          Pages: 8
          Original Paper

          Cardiovascular Medicine, Nephrology

          Nephrotic syndrome, Apo A-1, Intestinal, Gene expression


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