Blog
About

4
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Sildenafil inhibits the growth of human colorectal cancer in vitro and in vivo.

      Read this article at

      ScienceOpenPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Colorectal cancer is the third most common human cancer with frequent overexpression of the cGMP-specific phosphodiesterase 5 (PDE5). In the present study, we investigated that the anticancer effect of sildenafil on human colorectal cancer in vitro and in vivo, which is a potent and selective inhibitor of PDE5 for the treatment of erectile dysfunction and pulmonary arterial hypertension in the clinic. Sildenafil significantly induced cell growth inhibition, cell cycle arrest and apoptosis of human colorectal cancer with increased intracellular reactive oxidative specie (ROS) levels, which were accompanied by obvious alterations of related proteins such as CDKs, Cyclins and PARP etc. Pretreatment with ROS scavenger N-acetyl-L-cysteine could reverse sildenafil-induced ROS accumulation and cell apoptosis. Inhibition of the activity of protein kinase G with KT-5823 could enhance sildenafil-induced apoptosis. Furthermore, sildenafil caused the reduction of xenograft models of human colorectal cancer in nude mice. Overall, these findings suggest that sildenafil has the potential to be used for treatment of human colorectal cancer.

          Related collections

          Author and article information

          Journal
          Am J Cancer Res
          American journal of cancer research
          2156-6976
          2015
          : 5
          : 11
          Affiliations
          [1 ] Department of Cell Biology and Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
          [2 ] Department of Hepatobiliary Surgery, Cancer Center, Sun Yat-sen University Guangzhou 510060, Guangdong, China.
          [3 ] Department of Gastrointertinal Surgery and General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences Guangzhou 510080, Guangdong, China.
          [4 ] Department of Thoracic Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University Guangzhou 510060, Guangdong, China.
          Article
          4697679
          26807313

          colorectal cancer, PDE5, PKG, Sildenafil

          Comments

          Comment on this article