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Real-time quantification of different types of bcr-abl transcript in chronic myeloid leukemia.
M Amabile
1 ,
B Giannini ,
N Testoni ,
V Montefusco ,
G Rosti ,
C Zardini ,
C Terragna ,
S Buonamici ,
E Ottaviani ,
S Soverini ,
M Fiacchini ,
S Bassi ,
A de Vivo ,
E Trabacchi ,
G Saglio ,
F Pane ,
M Baccarani ,
S Tura ,
G Martinelli
Mar 2001
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Abstract
The most common translocation in chronic myeloid leukemia (CML) t(9;22) (q34;q22) produces the BCR/ABL fusion gene. We set up and evaluated a rapid and reliable real-time reverse-transcription-polymerase chain reaction (RT-PCR) approach using TaqMan technology for detection and quantification of bcr-abl transcripts in CML patients at diagnosis and during therapy.