Blog
About

10
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      In silico analysis of glutathione S-transferase supergene family revealed hitherto unreported insect specific δ- and ε-GSTs and mammalian specific μ-GSTs in Ixodes scapularis (Acari: Ixodidae).

      Computational Biology and Chemistry

      Phylogeny, Mammals, genetics, enzymology, Ixodes, Insects, Humans, Glutathione Transferase, Evolution, Molecular, Computational Biology, Animals, metabolism

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The availability of whole genome sequence information of Ixodes scapularis (Acari: Ixodidae), an important disease vector of veterinary and public health importance, has opened up new opportunities to explore the vector species at genomic level. Use of acaricides is the mainstay in controlling the disease vector, as effective vaccines are not available for most of the diseases that are transmitted by ticks. The glutathione S-transferase (GST) enzymes are one of the important supergene families that are involved in protecting the organism from oxidative stress and xenobiotics including the acaricides. The analysis of GST supergene family from Ixodes identified all the three broad GST classes, viz. canonical, mitochondrial, and microsomal forms. In total, 35 GST genes belong to five different canonical GST classes, namely Delta (7 genes), Epsilon (5), Mu (14), Omega (3), and Zeta (3 genes) GST classes, and two mitochondrial Kappa class GST genes, and a single microsomal GST gene were found. Interestingly, Delta- and Epsilon-class members, which are thought to be specific to the class Insecta, were also identified in Ixodes. Further, vertebrate/mammalian specific Mu-GSTs (14 genes) were also identified in Ixodes. Analyses of the intron-exon organization revealed higher frequency of phase '0' and phase '2' introns. The comprehensive listing of the GST supergene family members from Ixodes may help in understanding molecular mechanisms of the acaricide resistance in mites and ticks. Cumulatively, these findings may provide an in-depth understanding of the complex evolution of GST supergene family, one of the oldest supergene families that exist in all the domains of life. Copyright © 2011 Elsevier Ltd. All rights reserved.

          Related collections

          Author and article information

          Journal
          10.1016/j.compbiolchem.2011.03.004
          21514893

          Comments

          Comment on this article