7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Immunoextraction-tandem mass spectrometry method for measuring intact human chorionic gonadotropin, free β-subunit, and β-subunit core fragment in urine.

      1 , 2

      Clinical chemistry

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Human chorionic gonadotropin (hCG) stimulates testosterone production by the testicles. Because of the potential for abuse, hCG is banned (males only) in most sports and has been placed on the World Anti-Doping Agency list of prohibited substances. Intact hCG, free β-subunit (hCGβ), and β-subunit core fragment (hCGβcf) are the major variants or isoforms in urine. Immunoassays are used by antidoping laboratories to measure urinary hCG. Cross-reactivity with isoforms differs among immunoassays, resulting in widely varying results. We developed a sequential immunoextraction method with LC-MS/MS detection for quantification of intact hCG, hCGβ, and hCGβcf in urine.

          Related collections

          Author and article information

          Journal
          Clin. Chem.
          Clinical chemistry
          1530-8561
          0009-9147
          Aug 2014
          : 60
          : 8
          Affiliations
          [1 ] UCLA Olympic Analytical Laboratory, Department of Pathology & Laboratory Medicine, Geffen School of Medicine, Reagan UCLA Medical Center, Los Angeles, CA.
          [2 ] UCLA Olympic Analytical Laboratory, Department of Pathology & Laboratory Medicine, Geffen School of Medicine, Reagan UCLA Medical Center, Los Angeles, CA. abutch@mednet.ucla.edu.
          Article
          clinchem.2014.222703 NIHMS643562
          10.1373/clinchem.2014.222703
          4334569
          24899693
          © 2014 American Association for Clinical Chemistry.

          Comments

          Comment on this article