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      Fluoroprobe Quantification of Viable and Non-Viable Cells in Human Coronary and Internal Thoracic Arteries Sampled at Autopsy

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          Abstract

          Viable and non-viable cells in coronary and internal thoracic arteries, collected at autopsy 7-24 h post-mortem from individuals 15-81 years of age, were detected using the fïxable fluoroprobes 5-chloromethylfluorescein diacetate (green) and ethidium homodimer-1 (orange/red). Viability status of individual endothelial and smooth muscle cells was confirmed by simultaneous autoradiographic detection of incorporated [<sup>3</sup>H]glucosamine. Twenty-five percent of coronary and 42% of internal thoracic arteries contained viable cells up to 24 h following death. For the majority of viable vessels the mean percentage of viable cells ranged between 60 and 80% with no significant difference between coronary and internal thoracic arteries and no relationship with either age of the donor or with time to autopsy. Non-viable cells were usually distributed fairly evenly amongst viable cells but this pattern could not be assumed. In a number of vessels non-viable cells were variably clustered in different regions of vessel wall. These findings confirm that vessels sampled at autopsy can be used for metabolic studies with the caveat that assessment of cell viability is a necessary prerequisite for interpretation of results.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1995
          1995
          24 September 2008
          : 32
          : 6
          : 371-377
          Affiliations
          Department of Anatomy, School of Medicine, University of Auckland, New Zealand
          Article
          159112 J Vasc Res 1995;32:371–377
          10.1159/000159112
          8562809
          © 1995 S. Karger AG, Basel

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          Page count
          Pages: 7
          Categories
          Research Paper

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