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      cDNA cloning of four Hsp genes from Agarophyton vermiculophyllum and transcription analysis in different phases

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          Abstract

          Agarophyton vermiculophyllum is an agarophytic red alga originating from Northeast Asia that successfully spread to Europe and North America in the last 20 years. In this study, three hsp70 genes ( hsp70-1, hsp70-2, and hsp70-3) and one hsp90 gene were cloned, and real-time reverse transcription polymerase chain reaction (RT-qPCR) was used to measure their transcriptional levels in three kinds of thalli (tetrasporophytes, and male/female gametophytes) belonging to diploid or haploid phases in the life cycle of A. vermiculophyllum. The results show that the three Hsp70s in A. vermiculophyllum clustered into three different groups, and the locations of the putative Hsp70-1, Hsp70-2, and Hsp70-3 were in the cytoplasm, endoplasmic reticulum and chloroplast, respectively, according to their sequences. Beside on the same research, the putative Hsp90 was supposed to have a cytoplasmic location. The RT-qPCR results show that the three hsp70 genes were highly upregulated in gametophytes as compared to tetrasporophytes but the transcriptional level of hsp90 did not show such a significant increase. The chloroplast hsp70-3 exhibited the highest upregulation and the transcriptional level increased more than 570 fold in female gametophytes, and 17 fold in male gametophytes, compared with tetrasporophytes. Therefore, cpHsp70-3 might act more like a chaperon molecule involved in haploid development under natural condition, while Hsp70-1 and Hsp70-2 were more active in stress resistance.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 August 2020
          22 June 2020
          : 2
          : 3
          : 222-230
          Affiliations
          1The Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
          Author notes
          *Corresponding author: Di Xu, E-mail: dixu@ 123456ouc.edu.cn
          Article
          s42995-020-00049-9
          10.1007/s42995-020-00049-9
          © 2020 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
          Categories
          Research Paper

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