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      Fingerprinting genomes using PCR with arbitrary primers

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      Nucleic Acids Research
      Oxford University Press (OUP)

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          Abstract

          Simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (PCR). No prior sequence information is required. The method, arbitrarily primed PCR (AP-PCR), involves two cycles of low stringency amplification followed by PCR at higher stringency. We show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. The generality of the method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa (rice).

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          Author and article information

          Journal
          Nucleic Acids Research
          Nucl Acids Res
          Oxford University Press (OUP)
          0305-1048
          1362-4962
          1990
          1990
          : 18
          : 24
          : 7213-7218
          Article
          10.1093/nar/18.24.7213
          332855
          2259619
          53ab4cfe-e459-43b8-b84f-a370894e65fb
          © 1990
          History

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