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Abstract
<p class="first" id="d1225483e152">A glucose-sensitive antibacterial and anti-inflammatory
hydrogel film with controlled
release of tannic acid (TA) was synthesized using chitosan (CS). Specifically, the
photo-crosslinked CS hydrogel was first obtained and then immersed in TA solution
to generate composite hydrogel film with enhanced mechanical properties. Subsequently,
N-hydroxysuccinimide/1-ethyl-3-(3-dimethylaminopropyl) carbodiimide based coupling
chemistry was used to covalently crosslink glucose oxidase (GOx) to CS to obtain glucose
sensitivity. The physicochemical properties, including chemical composition, enzyme-related
characteristics, glucose responsiveness, and mechanical strength, were thoroughly
investigated, followed by the cytotoxicity, antibacterial and anti-inflammatory tests.
The results showed that the GOx immobilized on the film surface by covalent bonding
gave better stability than those that were physically adsorbed. In addition, it could
quickly and correspondingly modify its inner pore structure in response to the glucose
stimulus and then control the loaded TA release. Meanwhile, the TA addition could
enhance the film's mechanical properties. The composite hydrogel film demonstrated
adequate biocompatibility and can inhibit NO, IL-6, and TNF-α production in stimulated
macrophages, as well as Porphyromonas gingivalis growth, demonstrating effective antibacterial
and anti-inflammatory activity.
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